评价民间药用植物骆驼蓬的抗关节炎和抗炎潜力。
Appraisal of Anti-Arthritic and Anti-Inflammatory Potential of Folkloric Medicinal Plant Peganum harmala.
机构信息
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore Campus, Lahore, Pakistan.
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan.
出版信息
Endocr Metab Immune Disord Drug Targets. 2022;22(1):49-63. doi: 10.2174/1871530321666210208211310.
BACKGROUND & OBJECTIVE: Peganum harmala has been traditionally used to manage rheumatoid arthritis (RA) and other inflammatory conditions. However, its use against RA has not been scientifically evaluated. The current study was designed to assess the anti-arthritic and anti-inflammatory activities of the methanolic extract of P. harmala leaves by in vitro and in vivo methods.
METHODS
The in vitro assays were carried out to determine the effect of plant extract on inhibition of egg albumin denaturation and human red blood cell membrane (HRBC) stabilization. Moreover, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity was performed to determine the antioxidant potential. In vivo anti-arthritic activity was performed by determining the curative effect against Complete Freund's adjuvant (0.1 ml). The plant extract was administered to rats orally at 200, 400 and 600 mg/kg/day for 21 days.
RESULTS
The values of IC50 of plant extract in protein denaturation, stabilization of HRBC and DPPH assays were 77.54 mg/ml, 23.90 mg/ml and 58.09 μg/ml, respectively. Moreover, the plant extract significantly attenuated the poly-arthritis and weight loss, anemia and paw edema. The plant extract restored the level of C-reactive protein, rheumatoid factor, alanine transaminase, aspartate transaminase and alkaline phosphatase in poly-arthritic rats. Moreover, the plant extract restored the immune organs' weight in treated rats. Treatment with P. harmala also significantly subdued the oxidative stress by reinstating superoxide dismutase, reduced glutathione, catalase and malondialdehyde in poly-arthritic rats. The plant extract notably restored the prostaglandin-E2 and tumor necrosis factor (TNF)-α in the serum of poly-arthritic rats.
CONCLUSION
It was concluded that P. harmala extract had potential antioxidant, anti-inflammatory and antiarthritic activities, which primarily might be attributed to alkaloids, flavonoids and phenols.
背景与目的
骆驼蓬已被传统用于治疗类风湿关节炎（RA）和其他炎症性疾病。然而，其用于 RA 的疗效尚未得到科学评估。本研究旨在通过体外和体内方法评估骆驼蓬叶甲醇提取物的抗关节炎和抗炎活性。
方法
进行体外试验以确定植物提取物对抑制卵清蛋白变性和人红细胞膜（HRBC）稳定化的影响。此外，通过 2,2-二苯基-1-苦基肼（DPPH）清除活性测定来确定抗氧化潜力。通过测定对完全弗氏佐剂（0.1 ml）的疗效来进行体内抗关节炎活性。将植物提取物以 200、400 和 600 mg/kg/天的剂量经口给予大鼠 21 天。
结果
植物提取物在蛋白变性、HRBC 稳定化和 DPPH 试验中的 IC50 值分别为 77.54 mg/ml、23.90 mg/ml 和 58.09 μg/ml。此外，植物提取物显著减轻了多关节炎和体重减轻、贫血和爪水肿。植物提取物恢复了多关节炎大鼠的 C 反应蛋白、类风湿因子、丙氨酸转氨酶、天冬氨酸转氨酶和碱性磷酸酶水平。此外，植物提取物还恢复了治疗大鼠的免疫器官重量。骆驼蓬治疗还通过恢复超氧化物歧化酶、还原型谷胱甘肽、过氧化氢酶和丙二醛，显著抑制了多关节炎大鼠的氧化应激。植物提取物显著恢复了多关节炎大鼠血清中的前列腺素 E2 和肿瘤坏死因子（TNF）-α。
结论
骆驼蓬提取物具有潜在的抗氧化、抗炎和抗关节炎活性，这主要可能归因于生物碱、类黄酮和酚类化合物。
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