Department of Microbiology, Jahangirnagar University, Savar, Dhaka, Bangladesh.
Department of Zoology, Jahangirnagar University, Savar, Dhaka, Bangladesh.
J Med Virol. 2021 Jun;93(6):3564-3571. doi: 10.1002/jmv.26772. Epub 2021 Jan 12.
Acute gastroenteritis (AGE) is one of the most common diseases in children, and it continues to be a significant cause of morbidity and mortality worldwide. Norovirus is one of the major enteropathogens associated with both sporadic diarrhea and outbreaks of gastroenteritis. This study aims to investigate genotype diversity and molecular epidemiology of norovirus in Bangladesh. A total of 466 fecal specimens were collected from January 2014 to January 2019 from children below 5 years old with AGE in Bangladesh. All samples were analyzed by reverse transcriptase polymerase chain reaction to detect norovirus, and sequence analysis was conducted if found positive. Norovirus was detected in 5.1% (24 of 466) fecal specimens. Norovirus genotype GII.7 was predominant (62.5%, 15 of 24), followed by GII.3 (37.5%, 9 of 24). Coinfection between rotavirus and norovirus was found in 7 of 24 positive cases. Diarrhea (93.7%) and dehydration (89%) were the most common symptoms in children with AGE. About 80% of the positive cases were detected in children aged under 24 months. One seasonal peak (87.5% infection) was detected in the winter. This study suggests that norovirus continues to be one of the major etiologies of children AGE in Bangladesh. This study will provide a guideline to assess the burden of norovirus infection in Bangladesh, which will assist to combat against AGE.
急性胃肠炎（AGE）是儿童中最常见的疾病之一，它仍然是全球发病率和死亡率的重要原因。诺如病毒是与散发性腹泻和胃肠炎暴发相关的主要肠病原体之一。本研究旨在调查孟加拉国诺如病毒的基因型多样性和分子流行病学。从 2014 年 1 月至 2019 年 1 月，从孟加拉国 5 岁以下患有 AGE 的儿童中收集了总共 466 份粪便标本。所有样本均通过逆转录聚合酶链反应进行分析以检测诺如病毒，如果发现阳性则进行序列分析。在 466 份粪便标本中，有 5.1%（24 份）检测到诺如病毒。诺如病毒基因型 GII.7 占主导地位（62.5%，15 份），其次是 GII.3（37.5%，9 份）。在 24 个阳性病例中发现了轮状病毒和诺如病毒的混合感染。腹泻（93.7%）和脱水（89%）是 AGE 患儿最常见的症状。约 80%的阳性病例发生在 24 个月以下的儿童中。在冬季检测到一个季节性高峰（87.5%的感染）。本研究表明，诺如病毒仍然是孟加拉国儿童 AGE 的主要病因之一。本研究将为评估孟加拉国诺如病毒感染负担提供指导，这将有助于对抗 AGE。
