Chen Kun, Li Xiangyu, Wang Di, Ma Yanchun, Chen Bobin, Wang Qingping, Ma Jingjing, Guan Ming
Deptartment of Clinical Laboratory, Huashan Hospital North, Fudan University, Shanghai 201907, China.
Department of Hematology, Huashan Hospital North, Fudan University, Shanghai 201907, China.
Clin Chim Acta. 2021 Apr;515:21-26. doi: 10.1016/j.cca.2020.12.035. Epub 2020 Dec 31.
Vitreoretinal lymphoma (VRL) is a subtype of central lymphoma, which at present is hard to diagnose. The gold standard of VRL diagnosis is vitreous cytology, but the vitreous specimen needs to be obtained by the invasive surgery of vitrectomy. Aqueous humor is easier to obtain, has better stability, and has a lower operating risk than vitreous specimens.
We studied the diagnostic value of interleukin 10 (IL-10), IL-10/ IL-6 ratio and interleukin score for intraocular lymphoma diagnosis (ISOLD) in both vitreous and aqueous humor for vitreoretinal lymphoma, to determine if aqueous humor could be used to assist in the diagnosis of vitreoretinal lymphoma (VRL) by detecting IL-6 and IL-10.
The area under ROC curve (AUC) of vitreous fluid IL-10, IL-10/IL-6 ratio and ISOLD at the diagnosis of VRL diagnosis were 93.5%, 93.6% and 93.8%, respectively. The AUC of the aqueous humor IL-10, IL-10/IL-6 and ISOLD for VRL diagnosis were 83.8%, 72.8% and 85.9%, respectively.
Aqueous humor can thus replace vitreous humor as a potential sample type for the diagnosis of intraocular lymphoma.
玻璃体视网膜淋巴瘤（VRL）是中枢淋巴瘤的一种亚型，目前难以诊断。VRL诊断的金标准是玻璃体细胞学检查，但玻璃体标本需要通过玻璃体切割术这种侵入性手术获取。房水比玻璃体标本更容易获取，稳定性更好，手术风险更低。
我们研究了白细胞介素10（IL-10）、IL-10/IL-6比值和眼内淋巴瘤诊断白细胞介素评分（ISOLD）在玻璃体和房水中对玻璃体视网膜淋巴瘤的诊断价值，以确定通过检测IL-6和IL-10，房水是否可用于辅助诊断玻璃体视网膜淋巴瘤（VRL）。
玻璃体液IL-10、IL-10/IL-6比值和ISOLD在诊断VRL时的ROC曲线下面积（AUC）分别为93.5%、93.6%和93.8%。房水IL-10、IL-10/IL-6和ISOLD用于VRL诊断的AUC分别为83.8%、72.8%和85.9%。
因此，房水可替代玻璃体作为诊断眼内淋巴瘤的一种潜在样本类型。
