Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA.
Department of Biomedical Engineering, Northwestern University, Evanston, IL, USA; Center for Synthetic Biology, Northwestern University, Evanston, IL, USA; Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
Trends Biotechnol. 2021 Sep;39(9):927-939. doi: 10.1016/j.tibtech.2020.12.002. Epub 2020 Dec 30.
The combination of biological and synthetic materials has great potential to generate new types of biosensors. Toward this goal, recent advances in artificial cell development have demonstrated the capacity to detect a variety of analytes and environmental changes by encapsulating genetically encoded sensors within bilayer membranes, expanding the contexts within which biologically based sensing can operate. This chassis not only acts as a container for cell-free sensors, but can also play an active role in artificial cell sensing by serving as an additional gate mediating the transfer of environmental information. Here, we focus on recent progress toward stimuli-responsive artificial cells and discuss strategies for membrane functionalization in order to expand cell-free biosensing capabilities and applications.
生物材料和合成材料的结合具有产生新型生物传感器的巨大潜力。为了实现这一目标，最近在人工细胞开发方面的进展表明，通过将遗传编码的传感器封装在双层膜内，可以检测各种分析物和环境变化，从而扩展了生物传感可以运作的环境。这个底盘不仅可以作为无细胞传感器的容器，还可以通过充当额外的门控来介导环境信息的传递，在人工细胞传感中发挥积极作用。在这里，我们专注于对刺激响应型人工细胞的最新进展，并讨论了用于扩展无细胞生物传感能力和应用的膜功能化策略。
