Morgan B P
Department of Medical Biochemistry, University of Wales College of Medicine, Cardiff, U.K.
Immunology. 1988 Jan;63(1):71-7.
The metabolic consequences of non-lethal complement-membrane attack in neutrophils have been investigated by the measurement of cellular ATP content and functional parameters, including chemotactic and phagocytic responses and the capacity to secrete reactive oxygen metabolites, in cells before and after attack. Immediately after non-lethal complement attack, cellular ATP content was reduced by more than 75%, although lactate dehydrogenase content was unaltered. Energy-requiring cell functions were similarly depressed. Incubation of cells in nutrient medium rapidly restored cell-energy stores and functions, demonstrating the completeness of recovery. Fluorescence-activated cell-sorter studies demonstrated that cells undergoing non-lethal complement attack underwent a reversible cell swelling, the cell diameter rapidly increasing from an average of 8.5 micron to 12 micron, then gradually shrinking back to a final average diameter of 8.2 micron. The results indicate that although non-lethal complement-membrane attack causes both metabolic and physical changes in neutrophils, these effects are transient and full functional recovery occurs.
通过测量细胞攻击前后的细胞ATP含量和功能参数，包括趋化和吞噬反应以及分泌活性氧代谢物的能力，研究了中性粒细胞中非致死性补体膜攻击的代谢后果。非致死性补体攻击后，细胞ATP含量立即降低了75%以上，而乳酸脱氢酶含量未改变。需要能量的细胞功能同样受到抑制。在营养培养基中培养细胞可迅速恢复细胞能量储备和功能，表明恢复的完整性。荧光激活细胞分选仪研究表明，经历非致死性补体攻击的细胞发生了可逆的细胞肿胀，细胞直径从平均8.5微米迅速增加到12微米，然后逐渐缩小回最终平均直径8.2微米。结果表明，虽然非致死性补体膜攻击会导致中性粒细胞发生代谢和物理变化，但这些影响是短暂的，并且会发生完全的功能恢复。
