Enander I, Nygren H, Ahlstedt S
Pharmacia Diagnostics AB, Uppsala, Sweden.
Int Arch Allergy Appl Immunol. 1988;85(1):99-103. doi: 10.1159/000234481.
The suppression of delayed hypersensitivity (DH) in the lung as reflected by the appearance of mononuclear cells, mast cells and mucus-producing cells was studied in Balb/c mice. Immunosuppression was induced by intravenous and peroral administration of picrylsulfonic acid (PSA) in mice subsequently sensitized with picrylchloride (PiCl). The animals exhibited a decreased DH reactivity as assessed by ear thickness increase compared with mice sensitized but not exposed to PSA pretreatment. In mice exposed to PSA intravenously (suppressed) and sensitized with PiCl there was a decrease in the number of mononuclear cells in the lung after challenge, compared to mice sensitized and challenged only. Similarly, the mast cell and mucus-producing cell numbers were slightly lower in animals immunosuppressed intravenously with PSA compared with sensitized mice. Such a decrease in the numbers of mononuclear cells, mast cells and mucus-producing cells in the lung was not seen in animals treated with PSA perorally, although these animals exhibited suppressed DH reaction in the ears. The present results indicate that induction of mononuclear cells and to some extent mast cells and mucus-producing cells in the lung relates to the DH reaction and its regulation.
在Balb/c小鼠中，研究了用单核细胞、肥大细胞和黏液分泌细胞的出现所反映的肺部迟发型超敏反应（DH）的抑制情况。在用苦味酸氯（PiCl）致敏的小鼠中，通过静脉内和口服苦味磺酸（PSA）诱导免疫抑制。与仅致敏但未接受PSA预处理的小鼠相比，通过耳厚度增加评估，动物表现出DH反应性降低。在静脉内暴露于PSA（被抑制）并用PiCl致敏的小鼠中，与仅致敏和激发的小鼠相比，激发后肺中单核细胞数量减少。同样，与致敏小鼠相比，静脉内用PSA免疫抑制的动物中肥大细胞和黏液分泌细胞数量略低。在用PSA口服治疗的动物中，未观察到肺中单核细胞、肥大细胞和黏液分泌细胞数量的这种减少，尽管这些动物在耳部表现出DH反应受到抑制。目前的结果表明，肺中单核细胞以及在一定程度上肥大细胞和黏液分泌细胞的诱导与DH反应及其调节有关。
