Enander I, Ahlstedt S, Nygren H
Int Arch Allergy Appl Immunol. 1986;79(3):291-5. doi: 10.1159/000233989.
The appearance of mononuclear cells, mast cells and mucous cells in the airways was studied in Balb/c mice in relation to cell-mediated immunity reflected as delayed hypersensitivity (DH). The animals were pretreated with cyclophosphamide (Cy) to increase cell-mediated immunity and to decrease the influence of antibodies. Mice pretreated with Cy and epicutaneously sensitized with picrylchloride (PiCl) had stronger DH reactions as compared with sensitized mice not pretreated with Cy. The Cy treatment decreased the IgG antibody formation after sensitization. The Cy-pretreated, sensitized and challenged mice had increased numbers of mucus-producing cells and to some extent also mononuclear cells in their lungs compared with sensitized and challenged non-Cy-treated animals. However, the mucous cell numbers were also increased in Cy-treated, but nonsensitized mice in which most of the airway epithelium had differentiated into mucus-containing cells. The present results indicate that local cell-mediated immunity involves the appearance of mononuclear cells and mucus-producing cells in the lung. This inflammatory reaction is enhanced by Cy treatment, although mucous cell differentiation seems to be induced by exposure to Cy alone.
在Balb/c小鼠中，研究了气道中单核细胞、肥大细胞和黏液细胞的出现情况，并将其与作为迟发型超敏反应（DH）反映的细胞介导免疫相关联。用环磷酰胺（Cy）对动物进行预处理，以增强细胞介导免疫并减少抗体的影响。与未用Cy预处理的致敏小鼠相比，用Cy预处理并经皮用苦味酸氯（PiCl）致敏的小鼠具有更强的DH反应。Cy处理降低了致敏后IgG抗体的形成。与未用Cy处理的致敏和激发小鼠相比，经Cy预处理、致敏和激发的小鼠肺部产生黏液的细胞数量增加，单核细胞数量在一定程度上也增加。然而，在仅用Cy处理但未致敏的小鼠中，黏液细胞数量也增加，其中大部分气道上皮已分化为含黏液的细胞。目前的结果表明，局部细胞介导免疫涉及肺中单核细胞和产生黏液细胞的出现。这种炎症反应通过Cy处理而增强，尽管黏液细胞分化似乎仅由暴露于Cy诱导。
