Lang Lei, An De-Feng, Jiang Long-Qian, Li Gui-Ding, Wang Li-Song, Wang Xin-Yu, Li Qin-Yuan, Jiang Cheng-Lin, Jiang Yi
Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan University, Kunming, People's Republic of China.
Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People's Republic of China.
Curr Microbiol. 2021 Feb;78(2):816-821. doi: 10.1007/s00284-020-02321-8. Epub 2021 Jan 3.
Strain YIM 132242, isolated from lichen collected from Pu'er, Yunnan Province, China, was short-rod-shaped, Gram-reaction-negative, aerobic, catalase- and oxidase-positive. Growth of the strain was occurred at 10-39 °C (optimum, 28-35 °C), at pH 4.0-10.0 (optimum, pH 7.0-8.0) and at salinities of 0-8% (w/v) NaCl (optimum, 0-2%). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain YIM 132242 belonged to the genus Paracoccus and had the highest levels of sequence similarity to Paracoccus aerius KCTC 42845 (97.0% similarity), Paracoccus sediminis CMB17 (96.8% similarity), and Paracoccus fontiphilus MVW-1 (96.4% similarity). The major fatty acid was identified as C ω7c (77.6%). The predominant respiratory quinone was ubiquinone-10 (Q-10). Polar lipid analysis indicated the presence of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC), diphosphatidylglycerol (DPG), an unidentified lipid (L), and three unidentified phospholipids (PL1-PL3). Based on the draft genome sequence, the DNA G + C content of the strain was 67.1 mol%, and the values of average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) of strain YIM 132242 with Paracoccus aerius KCTC 42845 were 85.4% and 29.1%, respectively. On the basis of the data from this polyphasic characterization, strain YIM 132242 represents a novel species of the genus Paracoccus, for which the name Paracoccus lichenicola sp. nov. is proposed. The type strain is YIM 132242 (= KCTC 72463 = CGMCC1.17191).
从中国云南省普洱采集的地衣中分离出的菌株YIM 132242呈短杆状，革兰氏阴性，需氧，过氧化氢酶和氧化酶阳性。该菌株在10-39℃（最适温度为28-35℃）、pH 4.0-10.0（最适pH为7.0-8.0）和盐度0-8%（w/v）NaCl（最适盐度为0-2%）的条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株YIM 132242属于副球菌属，与嗜气副球菌KCTC 42845（相似度97.0%）、沉积副球菌CMB17（相似度96.8%）和嗜源副球菌MVW-1（相似度96.4%）的序列相似性最高。主要脂肪酸被鉴定为C ω7c（77.6%）。主要呼吸醌为泛醌-10（Q-10）。极性脂质分析表明存在磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）、二磷脂酰甘油（DPG）、一种未鉴定的脂质（L）和三种未鉴定的磷脂（PL1-PL3）。基于基因组草图序列，该菌株的DNA G+C含量为67.1mol%，菌株YIM 132242与嗜气副球菌KCTC 42845的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为85.4%和29.1%。基于多相特征分析的数据，菌株YIM 132242代表副球菌属的一个新物种，为此提出名为地衣副球菌（Paracoccus lichenicola sp. nov.）。模式菌株为YIM 132242（=KCTC 72463=CGMCC1.17191）。
