Key Laboratory for Conservation and Utilization of Bio-Resource, School of Life Sciences, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, People's Republic of China.
Institute of Microbial Pharmaceuticals, Northeastern University, Shenyang, 110819, People's Republic of China.
Arch Microbiol. 2020 Sep;202(7):1709-1715. doi: 10.1007/s00203-020-01881-4. Epub 2020 Apr 18.
A novel bacterial strain, designated YIM 132548, was isolated from Lepraria sp. lichen collected from Yunnan province, south-west PR China. The organism was Gram-stain negative, aerobic and methylotrophic. The cell was catalase positive and oxidase negative, asporogenous, rod-shaped and motile with three polar flagella. The strain could grow at 15-30 °C (optimum, 20 °C), at pH 6.0-9.0 (optimum, pH 7.0) and does not grow in the presence of NaCl. According to the 16S rRNA gene sequence analysis, strain YIM 132548 showed high levels of 16S rRNA gene sequence similarity with Methylobacterium soli YIM 48816 (97.6%) and Methylobacterium durans NBRC 112876 (97.3%), less than 97.0% with other validly named type strains of the genus Methylobacterium. Ubiquinone Q-10 was the predominant respiratory ubiquinone. The predominant cellular fatty acid was identified as summed feature 8 (Cω7c). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The DNA G + C content of the draft genome sequence is 70.2 mol%. The average nucleotide identity and digital DNA-DNA hybridizations values of strain YIM 132548 with M. soli YIM 48816 and M. durans NBRC 112876 were 87.0% and 82.0%, 40.6% and 27.2% based on draft genome sequences, respectively. On the basis of phylogenetic, chemotaxonomic, phenotypic and genomic data, strain YIM 132548 is concluded to represent a novel species of the genus Methylobacterium, for which the name Methylobacterium planium sp. nov. is proposed. The type strain is YIM 132548 (= CGMCC 1.17323 = NBRC 114056).
一株新的细菌菌株，命名为 YIM 132548，从中国西南部云南省采集的 Lepraria 地衣中分离得到。该生物为革兰氏阴性、需氧和甲基营养型。细胞呈过氧化氢酶阳性和氧化酶阴性，无孢子，杆状，有 3 根极生鞭毛，可运动。该菌株可在 15-30°C（最佳温度为 20°C）、pH 值 6.0-9.0（最佳 pH 值为 7.0）下生长，不能在 NaCl 存在的情况下生长。根据 16S rRNA 基因序列分析，菌株 YIM 132548 与 Methylobacterium soli YIM 48816（97.6%）和 Methylobacterium durans NBRC 112876（97.3%）的 16S rRNA 基因序列具有高度相似性，与其他有效命名的 Methylobacterium 属的模式菌株相似性低于 97.0%。泛醌 Q-10 是主要的呼吸泛醌。主要的细胞脂肪酸被鉴定为总和特征 8（Cω7c）。主要的极性脂是双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和磷脂酰胆碱。基因组草案序列的 DNA G+C 含量为 70.2mol%。菌株 YIM 132548 与 M. soli YIM 48816 和 M. durans NBRC 112876 的平均核苷酸同一性和数字 DNA-DNA 杂交值分别基于基因组草案序列为 87.0%和 82.0%、40.6%和 27.2%。基于系统发育、化学分类、表型和基因组数据，菌株 YIM 132548 被认为代表了 Methylobacterium 属的一个新种，建议将其命名为 Methylobacterium planium sp. nov.。模式菌株为 YIM 132548（=CGMCC 1.17323=NBRC 114056）。
