基于山奈酚的绿色合成银纳米粒子的抗菌活性的评价——对抗耐甲氧西林金黄色葡萄球菌
Critical Evaluation of Green Synthesized Silver Nanoparticles-Kaempferol for Antibacterial Activity Against Methicillin-Resistant .
机构信息
Department of Basic Sciences, Faculty of Dentistry, Universiti Sains Islam Malaysia, Kuala Lumpur, 55100, Malaysia.
Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Kepala Batas, Penang, 13200, Malaysia.
出版信息
Int J Nanomedicine. 2024 Feb 8;19:1339-1350. doi: 10.2147/IJN.S431499. eCollection 2024.
INTRODUCTION
This study aimed to characterize silver nanoparticles-kaempferol (AgNP-K) and its antibacterial activities against methicillin-resistant (MRSA). Green synthesis method was used to synthesize AgNP-K under the influence of temperature and different ratios of silver nitrate (AgNO and kaempferol).
METHODS
AgNP-K 1:1 was synthesized with 1 mM kaempferol, whereas AgNP-K 1:2 with 2 mM kaempferol. The characterization of AgNP-K 1:1 and AgNP-K 1:2 was performed using UV-visible spectroscopy (UV-Vis), Zetasizer, transmission electron microscopy (TEM), scanning electron microscopy-dispersive X-ray spectrometer (SEM-EDX), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. The antibacterial activities of five samples (AgNP-K 1:1, AgNP-K 1:2, commercial AgNPs, kaempferol, and vancomycin) at different concentrations (1.25, 2.5, 5, and 10 mg/mL) against MRSA were determined via disc diffusion assay (DDA), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) assay, and time-kill assay.
RESULTS
The presence of a dark brown colour in the solution indicated the formation of AgNP-K. The UV-visible absorption spectrum of the synthesized AgNP-K exhibited a broad peak at 447 nm. TEM, Zetasizer, and SEM-EDX results showed that the morphology and size of AgNP-K were nearly spherical in shape with 16.963 ± 6.0465 nm in size. XRD analysis confirmed that AgNP-K had a crystalline phase structure, while FTIR showed the absence of (-OH) group, indicating that kaempferol was successfully incorporated with silver. In DDA analysis, AgNP-K showed the largest inhibition zone (16.67 ± 1.19 mm) against MRSA as compared to kaempferol and commercial AgNPs. The MIC and MBC values for AgNP-K against MRSA were 1.25 and 2.50 mg/mL, respectively. The time-kill assay results showed that AgNP-K displayed bacteriostatic activity against MRSA. AgNP-K exhibited better antibacterial activity against MRSA when compared to commercial AgNPs or kaempferol alone.
简介
本研究旨在表征银纳米粒子-山柰酚（AgNP-K）及其对耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性。采用绿色合成方法，在温度和不同浓度的硝酸银（AgNO）和山柰酚的影响下合成 AgNP-K。
方法
用 1mM 山柰酚合成 AgNP-K1:1，用 2mM 山柰酚合成 AgNP-K1:2。用紫外-可见分光光度法（UV-Vis）、Zetasizer、透射电子显微镜（TEM）、扫描电子显微镜-能量色散 X 射线光谱仪（SEM-EDX）、X 射线衍射（XRD）和傅里叶变换红外光谱（FTIR）对 AgNP-K1:1 和 AgNP-K1:2 进行表征。采用纸片扩散法（DDA）、最小抑菌浓度（MIC）、最小杀菌浓度（MBC）测定和时间杀伤试验测定 5 种样品（AgNP-K1:1、AgNP-K1:2、商业 AgNPs、山柰酚和万古霉素）在不同浓度（1.25、2.5、5 和 10mg/mL）下对 MRSA 的抗菌活性。
结果
溶液呈深褐色表明 AgNP-K 的形成。合成的 AgNP-K 的紫外-可见吸收光谱在 447nm 处显示出一个宽峰。TEM、Zetasizer 和 SEM-EDX 结果表明，AgNP-K 的形态和尺寸几乎为球形，尺寸为 16.963±6.0465nm。XRD 分析证实 AgNP-K 具有结晶相结构，而 FTIR 显示（-OH）基团缺失，表明山柰酚已成功与银结合。在 DDA 分析中，AgNP-K 对 MRSA 的抑菌圈最大（16.67±1.19mm），优于山柰酚和商业 AgNPs。AgNP-K 对 MRSA 的 MIC 和 MBC 值分别为 1.25 和 2.50mg/mL。时间杀伤试验结果表明，AgNP-K 对 MRSA 具有抑菌活性。AgNP-K 对 MRSA 的抗菌活性优于单独的商业 AgNPs 或山柰酚。
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