Zhang Chan, Zhang Nan, Chen Mengxue, Wang Haijiao, Shi Jiachen, Wang Bei, Sun Baoguo, Wang Chengtao
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing, China.
Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology & Business University, Beijing, China.
Front Microbiol. 2020 Dec 17;11:610471. doi: 10.3389/fmicb.2020.610471. eCollection 2020.
Monacolin K is a secondary metabolite produced by with beneficial effects on health, including the ability to lower cholesterol. We previously showed that the yield of monacolin K was significantly improved when glutamic acid was added to the fermentation broth of M1. In this study, we analyzed in media with and without glutamic acid supplementation using a metabolomic profiling approach to identify key metabolites and metabolic pathway differences. A total of 817 differentially expressed metabolites were identified between the two fermentation broths on day 8 of fermentation. Pathway analysis of these metabolites using the KEGG database indicated overrepresentation of the citric acid cycle; biotin metabolism; and alanine, aspartate, and glutamate metabolic pathways. Six differentially expressed metabolites were found to be related to the citric acid cycle. The effect of citric acid as an exogenous additive on the synthesis of monacolin K was examined. These results provide technical support and a theoretical basis for further studies of the metabolic regulatory mechanisms underlying the beneficial effects of monacolin K and medium optimization, as well as genetic engineering of M1 for efficient monacolin K production.
莫纳可林K是由[具体菌种]产生的一种次生代谢产物，对健康具有有益作用，包括降低胆固醇的能力。我们之前表明，向[具体菌种]M1的发酵液中添加谷氨酸时，莫纳可林K的产量显著提高。在本研究中，我们使用代谢组学分析方法，分析了添加和未添加谷氨酸的培养基中的[具体菌种]，以确定关键代谢物和代谢途径差异。在发酵第8天，两种发酵液之间共鉴定出817种差异表达代谢物。使用KEGG数据库对这些代谢物进行通路分析表明，柠檬酸循环、生物素代谢以及丙氨酸、天冬氨酸和谷氨酸代谢途径存在过度表达。发现六种差异表达代谢物与柠檬酸循环有关。研究了柠檬酸作为外源添加剂对莫纳可林K合成的影响。这些结果为进一步研究莫纳可林K有益作用背后的代谢调控机制、培养基优化以及对[具体菌种]M1进行基因工程改造以高效生产莫纳可林K提供了技术支持和理论依据。
