Seck Waldiodio, Torkamaneh Davoud, Belzile François
Département de phytologie, Faculté des sciences de l'agriculture et de l'alimentation (FSAA), Université Laval, Quebec, QC, Canada.
Institut de biologie intégrative et des systèmes (IBIS), Université Laval, Quebec, QC, Canada.
Front Plant Sci. 2020 Dec 16;11:590740. doi: 10.3389/fpls.2020.590740. eCollection 2020.
Increasing the understanding genetic basis of the variability in root system architecture (RSA) is essential to improve resource-use efficiency in agriculture systems and to develop climate-resilient crop cultivars. Roots being underground, their direct observation and detailed characterization are challenging. Here, were characterized twelve RSA-related traits in a panel of 137 early maturing soybean lines (Canadian soybean core collection) using rhizoboxes and two-dimensional imaging. Significant phenotypic variation ( < 0.001) was observed among these lines for different RSA-related traits. This panel was genotyped with 2.18 million genome-wide single-nucleotide polymorphisms (SNPs) using a combination of genotyping-by-sequencing and whole-genome sequencing. A total of 10 quantitative trait locus (QTL) regions were detected for root total length and primary root diameter through a comprehensive genome-wide association study. These QTL regions explained from 15 to 25% of the phenotypic variation and contained two putative candidate genes with homology to genes previously reported to play a role in RSA in other species. These genes can serve to accelerate future efforts aimed to dissect genetic architecture of RSA and breed more resilient varieties.
增强对根系结构（RSA）变异性遗传基础的理解，对于提高农业系统中的资源利用效率以及培育适应气候变化的作物品种至关重要。由于根系生长在地下，对其进行直接观察和详细表征具有挑战性。在此，我们利用根箱和二维成像技术，对137个早熟大豆品系（加拿大大豆核心种质库）组成的群体中的12个与RSA相关的性状进行了表征。在这些品系中，不同的RSA相关性状均观察到显著的表型变异（<0.001）。利用简化基因组测序和全基因组测序相结合的方法，对该群体进行了218万个全基因组单核苷酸多态性（SNP）基因分型。通过全面的全基因组关联研究，共检测到10个与根总长和主根直径相关的数量性状位点（QTL）区域。这些QTL区域解释了15%至25%的表型变异，并包含两个与其他物种中先前报道的在RSA中起作用的基因具有同源性的推定候选基因。这些基因有助于加快未来剖析RSA遗传结构和培育更具适应性品种的研究进程。
