Lee N, Zhang S Q, Testa D
Genetic Systems Department, Interferon Sciences, Inc., New Brunswick, NJ 08901.
J Immunol Methods. 1988 Jan 21;106(1):27-30. doi: 10.1016/0022-1759(88)90267-0.
A multicolor Western blotting technique was developed, by which different kinds or different subtypes of interferon were identified with different colors on a single Western blot. This was achieved by sequentially applying different sets of probing antibodies, enzyme-conjugated developing antibodies and enzyme substrates to detect each of the two or more types of interferon on the Western blot. An improved and much faster method of obtaining the same result by the simultaneous application of more than one kind of probing antibodies, the simultaneous application of a mixture of different enzyme-conjugated developing antibodies followed by successive application of different substrates was also described. In addition, a combination of the sequential and simultaneous techniques was used to produce a triple color Western blot.
开发了一种多色蛋白质印迹技术，通过该技术可以在一张蛋白质印迹上用不同颜色鉴定不同种类或不同亚型的干扰素。这是通过依次应用不同组的检测抗体、酶联显色抗体和酶底物来检测蛋白质印迹上的两种或更多种干扰素中的每一种来实现的。还描述了一种改进的且速度更快的方法，即通过同时应用一种以上的检测抗体、同时应用不同酶联显色抗体的混合物，然后依次应用不同的底物来获得相同的结果。此外，还使用了顺序和同时技术的组合来制作三色蛋白质印迹。
