Biotechnology Department, Nong Lam University HCMC, Linh Trung ward, Thu Duc district, Ho Chi Minh, Vietnam.
School of Medicine, Vietnam National University Ho Chi Minh City, Linh Trung ward, Thu Duc district, Ho Chi Minh, Vietnam.
Arch Virol. 2021 Feb;166(2):403-411. doi: 10.1007/s00705-020-04878-y. Epub 2021 Jan 3.
This study was conducted to investigate the genetic diversity of porcine circovirus type 2 (PCV2) and its coinfecting pathogens in pigs with respiratory disease in Vietnam. Samples from 127 clinical cases were obtained from different southern provinces of Vietnam from January 2018 to January 2020 for PCR and sequence analysis. The infection rate of PCV2 was 78.8%, and the major pathogens found in coinfections with PCV2 were porcine reproductive and respiratory syndrome virus, Mycoplasma hyopneumoniae, and Haemophilus parasuis. Forty-three PCV2-positive clinical samples were selected for amplification and sequencing of the ORF2 region. Phylogenetic analysis of PCV2 ORF2 showed that five of the sequences belonged to PCV2b (11.6%) and 38 belonged to PCV2d (88.4%), indicating that PCV2d strains were predominant in southern provinces of Vietnam. Alignment of the predicted amino acid sequences of the PCV2 capsid protein revealed polymorphic sites in the antibody recognition regions. This study demonstrates the prevalence of the PCV2d genotype in southern Vietnam and presents a comprehensive overview of the coinfecting pathogens associated with PCV2 in young pigs with respiratory disease.
本研究旨在调查越南患有呼吸道疾病的猪中猪圆环病毒 2 型（PCV2）及其合并感染病原体的遗传多样性。2018 年 1 月至 2020 年 1 月，从越南南部不同省份的 127 个临床病例中采集样本，进行 PCR 和序列分析。PCV2 的感染率为 78.8%，与 PCV2 合并感染的主要病原体为猪繁殖与呼吸综合征病毒、猪肺炎支原体和副猪嗜血杆菌。从 43 份 PCV2 阳性临床样本中选择 ORF2 区进行扩增和测序。PCV2 ORF2 的系统发育分析表明，其中 5 个序列属于 PCV2b（11.6%），38 个序列属于 PCV2d（88.4%），表明 PCV2d 株在越南南部省份占优势。PCV2 衣壳蛋白预测氨基酸序列的比对显示在抗体识别区域存在多态性位点。本研究证明了 PCV2d 基因型在越南南部的流行，并全面概述了与患有呼吸道疾病的年轻猪中 PCV2 合并感染的病原体。
