Department of Chemistry, Yunnan Normal University, Kunming 650500, China.
J Chromatogr Sci. 2021 Mar 19;59(4):355-360. doi: 10.1093/chromsci/bmaa117.
A homochiral metal-organic framework (MOF) comprising [Co(L)(bpe)2(H2O)2]·H2O was prepared using (1R,2R)-(-)-1,2-cyclohexanedicarboxylic acid (H2L) and 1,2-bis(4-pyridyl)-ethylene as organic ligands. The homochiral MOF [Co(L)(bpe)2(H2O)2]·H2O was explored as chiral stationary phase (CSP) for high-performance liquid chromatography (HPLC) separation of racemates. Nine racemates including naphthol, alcohol, diol, amine, ketone, ether and organic acid were well separated on the homochiral MOF [Co(L)(bpe)2(H2O)2]·H2O column (250 mm long × 4.6 mm i.d.). The relative standard deviation for five replicate separations of 1,1'-bi-2-naphthol is 0.69% for the retention time, indicating that the good reproducibility and stability of the homochiral MOF column for HPLC enantioseparation. The results indicated that the homochiral MOF as CSP is practical, which promotes the application of homochiral MOFs in HPLC.
一种手性金属-有机骨架（MOF）由[Co（L）（bpe）2（H2O）2]·H2O 组成，使用（1R，2R）-（-）-1,2-环己烷二甲酸（H2L）和 1,2-双（4-吡啶基）-乙烯作为有机配体。手性 MOF [Co（L）（bpe）2（H2O）2]·H2O 被探索用作高效液相色谱（HPLC）拆分外消旋体的手性固定相（CSP）。9 种外消旋体，包括萘酚、醇、二醇、胺、酮、醚和有机酸，在手性 MOF [Co（L）（bpe）2（H2O）2]·H2O 柱上得到了很好的分离（250mm 长×4.6mm id）。五种 1,1'-双-2-萘酚拆分重复分离的保留时间的相对标准偏差为 0.69%，表明手性 MOF 柱用于 HPLC 对映体拆分具有良好的重现性和稳定性。结果表明，手性 MOF 作为 CSP 是实用的，这促进了手性 MOF 在 HPLC 中的应用。
