AlJindan Reem, AlEraky Doaa M, Mahmoud Nehal, Abdalhamid Baha, Almustafa Mashael, AbdulAzeez Sayed, Borgio J Francis
Department of Microbiology and Immunology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam 40017, Saudi Arabia.
Department of Biomedical Dental Science, Microbiology and Immunology Division, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 40344, Saudi Arabia.
J Fungi (Basel). 2020 Dec 31;7(1):18. doi: 10.3390/jof7010018.
is an emerging multi-drug resistant pathogen with high mortality rate; nosocomial infections have been reported worldwide, causing a major challenge for clinicians and microbiological laboratories. The study aims to describe new cases of and detect drug resistance-associated mutations of by the sequencing of and genes. A total of six specimens were collected from blood, urine, ear swab, and groin screening samples. Isolates were incubated for 48 h on Sabouraud Dextrose agar (SDA) at 42 °C, then confirmed by MALDI-TOF MS. Furthermore, antifungal susceptibility testing was performed using the Vitek 2 system to detect Minimum Inhibitory Concentrations (MICs) of six antifungals. Sequences of gene and ITS regions from isolates and phylogenetic analysis were performed. Gene sequencing was analysed to detect drug resistance-associated mutations by and genes sequencing. All isolates were confirmed by MALDI-TOF MS, and evolutionary analyses using sequences of gene and ITS region. Antifungal susceptibility testing showed that all isolates were resistant to fluconazole. Sequencing of and genes from the isolates revealed the presence of two (F132Y and K143R) drug resistance-associated mutations in , however, gene was devoid of mutations. The study sheds light on a public health threat of an emerging pathogen, and the hospital implemented strict contact screening and infection control precautions to prevent infection. Finally, there is a critical need to monitor the antifungal resistance in different geographical areas and implementation of efficient guidelines for treatment.
是一种新兴的具有高死亡率的多重耐药病原体；全球范围内均有医院感染的报道，这给临床医生和微生物实验室带来了重大挑战。本研究旨在描述新的病例，并通过对基因和基因进行测序来检测与耐药相关的突变。共从血液、尿液、耳部拭子和腹股沟筛查样本中采集了六个标本。分离株在42℃的沙氏葡萄糖琼脂（SDA）上孵育48小时，然后通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）进行鉴定。此外，使用Vitek 2系统进行抗真菌药敏试验，以检测六种抗真菌药物的最低抑菌浓度（MIC）。对分离株的基因和ITS区域进行测序及系统发育分析。通过对基因和基因进行测序来分析基因序列，以检测与耐药相关的突变。所有分离株均通过MALDI-TOF MS进行鉴定，并利用基因和ITS区域的序列进行进化分析。抗真菌药敏试验表明，所有分离株均对氟康唑耐药。对分离株的基因和基因进行测序发现，基因中存在两个（F132Y和K143R）与耐药相关的突变，然而，基因未发现突变。该研究揭示了一种新兴病原体对公共卫生的威胁，医院实施了严格的接触筛查和感染控制预防措施以防止感染。最后，迫切需要监测不同地理区域的抗真菌耐药情况，并实施有效的治疗指南。
