Rapid Detection of -Associated Azole Resistance and -Associated Echinocandin Resistance in Candida auris.

is an emerging multidrug-resistant yeast that can cause serious invasive infections. The accurate and rapid assessment of antifungal resistance is important for effective patient management. A novel and highly accurate diagnostic platform was established for the rapid identification of mutations conferring azole resistance and mutations associated with echinocandin resistance in Using allele-specific molecular beacons and DNA melting curve analysis following asymmetric PCR, a duplex assay and a simplex HS1 assay were developed to identify the most prominent resistance-associated mutations (Y132F and K143R in ; S639F in HS1) within 2 h. Assays were validated by testing a panel of 94 clinical isolates in a blind manner. The molecular diagnostic results from the assays were 100% concordant with DNA sequencing results. This platform has the potential to overcome the deficiencies of existing susceptibility-based assays to identify azole- and/or echinocandin-resistant , and thus, it holds promise as a surrogate diagnostic method to direct antifungal therapy more effectively.