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一种超灵敏技术：使用线粒体细胞色素c氧化酶亚基1定量聚合酶链反应检测巴西亚马逊地区患者的早期复发情况

An Ultra-Sensitive Technique: Using -mtCOX1 qPCR to Detect Early Recurrences of in Patients in the Brazilian Amazon.

作者信息

Barbosa Laila R A, da Silva Emanuelle L, de Almeida Anne C G, Salazar Yanka E A R, Siqueira André M, Alecrim Maria das Graças Costa, Vieira José Luiz Fernandes, Bassat Quique, de Lacerda Marcus V G, Monteiro Wuelton M, Melo Gisely C

机构信息

Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Instituto de Pesquisa Clínica Carlos Borborema, Manaus 69040-200, AM, Brazil.

Programa de Pós-Graduação em Medicina Tropical, Universidade do Estado do Amazonas, Manaus 69040-200, AM, Brazil.

出版信息

Pathogens. 2020 Dec 30;10(1):19. doi: 10.3390/pathogens10010019.

DOI:10.3390/pathogens10010019

PMID:33396824

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7823330/

Abstract

BACKGROUND

Early recurrence of is a challenge for malaria control in the field, particularly because this species is associated with lower parasitemia, which hinders diagnosis and monitoring through blood smear testing. Early recurrences, defined as the persistence of parasites in the peripheral blood despite adequate drug dosages, may arise from resistance to chloroquine. The objective of the study was to estimate early recurrence of in the Brazilian Amazon by using a highly-sensitive detection method, in this case, PCR.

METHODS

An ultra-sensitive qPCR that targeted mitochondrial DNA was used to compare a standard qPCR that targeted 18S rDNA to detect early recurrence of in very low densities in samples from patients treated with chloroquine.

RESULTS

Out of a total of 312 cases, 29 samples (9.3%) were characterized as recurrences, from which 3.2% (10/312) were only detected through ultra-sensitive qPCR testing.

CONCLUSIONS

Studies that report the detection of early recurrences using light microscopy may severely underestimate their true incidence.

摘要

背景

间日疟原虫的早期复发是疟疾现场防控面临的一项挑战，特别是因为该物种与较低的寄生虫血症相关，这会妨碍通过血涂片检测进行诊断和监测。早期复发定义为尽管药物剂量充足但外周血中仍存在寄生虫，可能是由于对氯喹产生耐药性所致。本研究的目的是通过使用一种高灵敏度检测方法（在本案例中为聚合酶链反应（PCR））来估计巴西亚马逊地区间日疟原虫的早期复发情况。