通过辅因子代谢工程策略调控紫红红曲霉菌中次生代谢产物的生物合成
Regulation of secondary metabolite biosynthesis in Monascus purpureus via cofactor metabolic engineering strategies.
作者信息
Liu Jun, Wu Jingyan, Cai Xinru, Zhang Song, Liang Ying, Lin Qinlu
机构信息
National Engineering Laboratory for Deep Process of Rice and By-products, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.
National Engineering Laboratory for Deep Process of Rice and By-products, Hunan Key Laboratory of Grain-oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China.
出版信息
Food Microbiol. 2021 May;95:103689. doi: 10.1016/j.fm.2020.103689. Epub 2020 Nov 23.
This study investigated the effects of cofactor metabolism on secondary metabolite production in M. purpureus through the application of different cofactor engineering strategies. Total pigment production dramatically increased by 39.08% and 40.89%, and yellow pigment production increased by 74.62% and 114.06% after the addition of 1.0 mg/L of the exogenous cofactor reagents methyl viologen and rotenone, respectively, in submerged batch-fermentation. The extracellular red pigment tone changed to yellow with the application of electrolytic stimulation at 800 mV/cm, but almost no citrinin production was detected. In addition, the total pigment, yellow pigment and citrinin production increased by 35.46%, 54.89% and 6.27% after disruption of the nuoⅠ gene that encodes NADH-quinone oxidoreductase, respectively. Thus, cofactor metabolic engineering strategies could be extended to the industrial production of Monascus pigment or high yellow pigment with free citrinin production.
本研究通过应用不同的辅因子工程策略，研究了辅因子代谢对紫红曲霉次级代谢产物产生的影响。在深层分批发酵中，分别添加1.0 mg/L的外源辅因子试剂甲基紫精和鱼藤酮后，总色素产量显著增加了39.08%和40.89%，黄色色素产量分别增加了74.62%和114.06%。在800 mV/cm的电解刺激下，细胞外红色色素色调变为黄色，但几乎未检测到桔霉素产生。此外，在编码NADH-醌氧化还原酶的nuoⅠ基因被破坏后，总色素、黄色色素和桔霉素产量分别增加了35.46%、54.89%和6.27%。因此，辅因子代谢工程策略可扩展到红曲色素或无桔霉素产生的高黄色色素的工业生产中。
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