Yi Zhigao, All Angelo H, Liu Xiaogang
Department of Chemistry, National University of Singapore, Singapore, Singapore.
Department of Chemistry, Faculty of Science, Hong Kong Baptist University, Kowloon, Hong Kong.
Adv Exp Med Biol. 2021;1293:641-657. doi: 10.1007/978-981-15-8763-4_44.
Upconversion nanoparticle-mediated optogenetics enables remote delivery of upconverted visible light from a near-infrared light source to targeted neurons or areas, with the precision of a pulse of laser light in vivo for effective deep-tissue neuromodulation. Compared to conventional optogenetic tools, upconversion nanoparticle-based optogenetic techniques are less invasive and cause reduced inflammation with minimal levels of tissue damage. In addition to the optical stimulation, this design offers simultaneously temperature recording in proximity to the stimulated area. This chapter strives to provide life science researchers with an introduction to upconversion optogenetics, starting from the fundamental concept of photon upconversion and nanoparticle fabrication to the current state-of-the-art of surface engineering and device integration for minimally invasive neuromodulation.
上转换纳米颗粒介导的光遗传学能够将来自近红外光源的上转换可见光远程传递到目标神经元或区域，在体内实现激光脉冲的精度，以进行有效的深部组织神经调节。与传统的光遗传学工具相比，基于上转换纳米颗粒的光遗传学技术侵入性较小，炎症反应减轻，组织损伤水平最低。除了光刺激外，这种设计还能在受刺激区域附近同时进行温度记录。本章致力于为生命科学研究人员介绍上转换光遗传学，从光子上转换的基本概念和纳米颗粒制造，到目前用于微创神经调节的表面工程和器件集成的最新技术。
