Department of Biomaterials and Cosmetic Chemistry, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarin 7, 87-100 Torun, Poland.
Department of Cell Biology and Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Kraków, Gronostajowa 7, 30-387 Kraków, Poland.
IET Nanobiotechnol. 2020 Dec;14(9):830-832. doi: 10.1049/iet-nbt.2020.0045.
Scaffolds based on chitosan (CTS), collagen (Coll) and glycosaminoglycans (GAG) mixtures cross-linked by tannic acid (TA) with bioglass 45S5 addition were obtained with the use of the freeze-drying method. The prepared scaffolds were characterised for morphology, mechanical strength and degradation rate. Moreover, cell viability on the obtained scaffolds was measured with and without the presence of ascorbic acid and dexamethasone. The main purpose of the research was to compare the effectiveness of bioglass 45S5 influence on the physicochemical and biological properties of scaffolds. The results demonstrated that the scaffolds based on the blends of biopolymers cross-linked by TA are stable in an aqueous environment. Scanning electron microscope images allowed the observation of a porous scaffold structure with interconnected pores. The addition of bioglass nanoparticles improved the mechanical properties and decreased the degradation rate of composite materials. The biological properties were improved for 20% tannic acid addition compared to 5%. However, the addition of bioglass 45S5 did not change to cells response significantly.
基于壳聚糖 (CTS)、胶原蛋白 (Coll) 和糖胺聚糖 (GAG) 混合物的支架，通过单宁酸 (TA) 交联，并添加生物玻璃 45S5，使用冷冻干燥法获得。对制备的支架进行了形态、力学强度和降解率的特性研究。此外，还在有/无抗坏血酸和地塞米松存在的情况下，测量了细胞在获得的支架上的存活率。研究的主要目的是比较生物玻璃 45S5 对支架理化和生物学性质的影响。结果表明，基于 TA 交联的生物聚合物混合物的支架在水环境中稳定。扫描电子显微镜图像允许观察到具有相互连接孔的多孔支架结构。添加纳米生物玻璃提高了复合材料的机械性能并降低了降解率。与 5%相比，添加 20%单宁酸可提高生物性能。然而，生物玻璃 45S5 的添加并没有显著改变细胞的反应。
