Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711103, West Bengal, India.
Department of Aerospace Engineering and Applied Mechanics, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711103, West Bengal, India.
IET Nanobiotechnol. 2020 Dec;14(9):870-880. doi: 10.1049/iet-nbt.2019.0288.
Umbilical cord-derived mesenchymal stem cells (UCDMSC) are attractive candidates for cell-based regenerative medicine. However, they are susceptible to replicative senescence during repetitive passaging for in-vitro expansion and induced senescence in an oxidative, inflammatory microenvironment in vivo. Aim of this study is to investigate if honey-incorporated matrices can be employed to reduce senescence of UCDMSC. Matrices were prepared by electrospinning solutions of honey with poly-vinyl alcohol (PVA). PVA:honey matrices exhibited free radical scavenging activity. Culture of UCDMSC on PVA:honey matrices showed improvement in cell proliferation compared to pure PVA nanofibres. Expression of vimentin indicated that mesenchymal phenotype is preserved after culturing on these matrices. Further, UCDMSC were serially subcultured and cells of two passages (P2 and P6) were evaluated for reactive oxygen species (ROS) load and senescence parameters. P6 cells showed a higher ROS load and β-galactosidase (β-gal) positive senescent cells compared to P2. However, culturing on PVA:honey substrates significantly reduced both ROS and β-gal markers compared to cells on PVA substrates. Honey contains several antioxidant and anti-inflammatory components, which can reduce the ROS-related senescence. Thus, it is concluded that honey containing nanofibres can be effective substrates for stem cell-based wound healing and regenerative medicine.
脐带间充质干细胞（UCDMSC）是细胞再生医学中很有吸引力的候选细胞。然而，在体外扩增过程中进行反复传代时，它们易发生复制性衰老，在体内氧化、炎症的微环境中会诱导衰老。本研究旨在探讨是否可以使用含有蜂蜜的基质来减少 UCDMSC 的衰老。通过静电纺丝含有聚已醇（PVA）的蜂蜜溶液来制备基质。PVA：蜂蜜基质表现出自由基清除活性。与纯 PVA 纳米纤维相比，UCDMSC 在 PVA：蜂蜜基质上培养显示出细胞增殖的改善。波形蛋白的表达表明，在这些基质上培养后保留了间充质表型。此外，对 UCDMSC 进行连续传代，评估两代（P2 和 P6）细胞的活性氧（ROS）负荷和衰老参数。与 P2 相比，P6 细胞显示出更高的 ROS 负荷和β-半乳糖苷酶（β-gal）阳性衰老细胞。然而，与在 PVA 基质上的细胞相比，在 PVA：蜂蜜基质上培养显著降低了两种 ROS 和β-gal 标志物。蜂蜜含有几种抗氧化和抗炎成分，可减少与 ROS 相关的衰老。因此，可以得出结论，含有蜂蜜的纳米纤维可以作为基于干细胞的伤口愈合和再生医学的有效基质。
