Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University.
Family medicine department, Faculty of Medicine, Cairo University.
Afr Health Sci. 2020 Sep;20(3):1283-1291. doi: 10.4314/ahs.v20i3.33.
Colorectal cancer (CRC) has high morbidity and mortality rates. Invasive techniques and other laboratory tests with variable sensitivity and specificity are currently used in diagnosis. Micro ribonucleic acids (miRNAs) have bio vital roles in cell proliferation and apoptosis. Dys-regulation of miRNAs is linked to tumour genesis. The objective of this study was to evaluate the specificity and sensitivity of serum non-invasive biomarkers (micro-RNAs), miR-1246, miR-23a, and miR-451in CRC patients.
Peripheral expression of three miRNAs (miR-1246, miR-23a and miR-451) was investigated in sera of 37 CRC Egyptian patients and 30 healthy controls, using quantitative real-time polymerase chain reaction trying to reach the optimal non-invasive combination of miRNAs.
Serum miR-1246 was up-regulated in sera of CRC patients compared to normal controls (fold change = 3.55; P<0.001) and showed 100% sensitivity and 80% specificity in diagnosis of CRC. Serum miR-451 was significantly down-regulated in CRC patients (fold change = -4.86; p= 0.014), whereas, miR-23a was down-regulated but this was not statistically significant.
Up-regulation of miR-1246 and down-regulation of miR-451 in the sera of primary CRC Egyptian patients were confirmed with high sensitivity and specificity. Large-scale studies on a wider spectrum of miRNAs in Egyptian CRC patients are needed.
结直肠癌（CRC）的发病率和死亡率都很高。目前，在诊断中使用了侵入性技术和其他具有可变灵敏度和特异性的实验室检测。微小核糖核酸（miRNAs）在细胞增殖和凋亡中具有重要的生物学作用。miRNAs 的失调与肿瘤发生有关。本研究旨在评估血清非侵入性生物标志物（miRNAs）miR-1246、miR-23a 和 miR-451 在 CRC 患者中的特异性和敏感性。
使用定量实时聚合酶链反应检测 37 例埃及 CRC 患者和 30 例健康对照者血清中三种 miRNA（miR-1246、miR-23a 和 miR-451）的外周表达，试图达到最佳的非侵入性 miRNA 组合。
与正常对照组相比，CRC 患者血清 miR-1246 上调（倍数变化=3.55；P<0.001），对 CRC 的诊断具有 100%的敏感性和 80%的特异性。CRC 患者血清 miR-451 显著下调（倍数变化=-4.86；p=0.014），而 miR-23a 下调但无统计学意义。
原发性埃及 CRC 患者血清中 miR-1246 的上调和 miR-451 的下调得到了证实，具有较高的敏感性和特异性。需要在埃及 CRC 患者中进行更大规模的研究，以研究更广泛的 miRNA 谱。
