Xu Jianbo, Ou Xinde, Li Jin, Cai Qinbo, Sun Kaiyu, Ye Jingning, Peng Jianjun
Department of Gastrointestinal Surgery, the First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2nd Road, Guangzhou 510080, China.
Laboratory of General Surgery, the First Affiliated Hospital, Sun Yat-sen University, 58 Zhongshan 2nd Road, Guangzhou 510080, China.
J Cancer. 2021 Jan 1;12(3):807-817. doi: 10.7150/jca.50653. eCollection 2021.
Tac2-N (TC2N) is a tandem C2 domain-containing protein, acting as a novel oncogene or suppressor in different kinds of cancers. However, the status of expression and its significance in gastric cancer (GC) is still unclear. The present study is aimed to elucidate the clinicopathological significance and prognostic value of level in GC. We used sequencing data from the Cancer Genome Atlas (TCGA) database to analyze expression in GC by UALCAN database and Gene Expression Profiling Interactive Analysis tools (GEPIA). expression level in 12 pairs of fresh GC tissues and adjacent nontumorous tissues was detected by quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) and Western blot (WB) assays. Immunohistochemical (IHC) staining was used to detect TC2N protein expression in Paraffin-embedded tissues in our center. proliferation, migration and invasion assays were used to evaluate the effect of on functional capability of gastric cancer cells. LinkedOmics was used to identify gene expressions associated with . The mRNA and protein expression of in gastric cancer were both significantly higher than normal gastric mucosa. It was also elevated in gastric cancer cells compared with normal gastric epithelium cell. assays suggested that facilitated proliferation, migration and invasion of gastric cancer cells. Bioinformatic analysis showed a widespread impact of on the transcriptome and a strong interaction with tumor associated genes. We also found that was an independent prognostic factor for long-term survival in GC patients and its high expression was evidently associated with poor overall survival and recurrence-free survival. Our results show that high level of TC2N correlates with poor prognosis in patients with gastric cancer and promotes the development of gastric cancer. Thus, TC2N expression can serve as a prognostic biomarker for patients with gastric cancer.
Tac2-N（TC2N）是一种含有串联C2结构域的蛋白质，在不同类型的癌症中作为一种新型癌基因或抑癌基因发挥作用。然而，其在胃癌（GC）中的表达状况及其意义仍不清楚。本研究旨在阐明TC2N在GC中的临床病理意义和预后价值。我们使用来自癌症基因组图谱（TCGA）数据库的测序数据，通过UALCAN数据库和基因表达谱交互式分析工具（GEPIA）分析GC中TC2N的表达。通过定量实时逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹（WB）检测12对新鲜GC组织和相邻非肿瘤组织中TC2N的表达水平。免疫组织化学（IHC）染色用于检测我们中心石蜡包埋组织中TC2N蛋白的表达。采用增殖、迁移和侵袭实验来评估TC2N对胃癌细胞功能能力的影响。使用LinkedOmics来鉴定与TC2N相关的基因表达。TC2N在胃癌中的mRNA和蛋白表达均显著高于正常胃黏膜。与正常胃上皮细胞相比，其在胃癌细胞中也升高。实验表明TC2N促进了胃癌细胞的增殖、迁移和侵袭。生物信息学分析显示TC2N对转录组有广泛影响，并与肿瘤相关基因有强烈相互作用。我们还发现TC2N是GC患者长期生存的独立预后因素，其高表达明显与总体生存率差和无复发生存率差相关。我们的结果表明，TC2N高水平与胃癌患者预后不良相关，并促进胃癌的发展。因此，TC2N表达可作为胃癌患者的预后生物标志物。
