Kim Sooah, Kim Jungyeon, Kim Nahyun, Lee Dongho, Lee Hojoung, Lee Dong-Yup, Kim Kyoung Heon
Department of Environment Science and Biotechnology, Jeonju University, Jeonju 55069, South Korea.
Department of Biotechnology, Graduate School, Korea University, Seoul 02841, South Korea.
ACS Omega. 2020 Dec 15;5(51):33186-33195. doi: 10.1021/acsomega.0c04745. eCollection 2020 Dec 29.
Sucrose induces flavonoid accumulation in plants as a defense mechanism against various stresses. However, the relationship between the biosynthesis of flavonoids as secondary metabolites and sucrose levels remains unknown. To understand the change in flavonoid biosynthesis by sucrose, we conducted secondary metabolite profiling in treated with different levels of sucrose using ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry. The partial least squares-discriminant and hierarchical clustering analyses showed significant differences in secondary metabolite profiles in at 50, 150, and 300 mM sucrose levels. The levels of 3 flavonoids such as quercetin 3--β-d-glucosyl-(1→2)-β-d-glucoside, 6-methoxyaromadendrin 3--acetate, and 3-hydroxycoumarin and 19 flavonoids including 6-methoxyaromadendrin 3--acetate, aureusidin, iridin, flavonol 3--(6--malonyl-β-d-glucoside) quercetin 3--glucoside, and rutin increased at 150 and 300 mM sucrose, respectively, compared to 50 mM sucrose, indicating that the flavonoids were accumulated in by a higher concentration of sucrose. This is the first investigation of the change in individual flavonoids as secondary metabolites in by varying sucrose levels, and the results demonstrate that the sucrose causes the accumulation of certain flavonoids as a defense mechanism against osmotic stress.
蔗糖可诱导植物中黄酮类化合物的积累，作为抵御各种胁迫的防御机制。然而，作为次生代谢产物的黄酮类化合物生物合成与蔗糖水平之间的关系仍不清楚。为了了解蔗糖对黄酮类化合物生物合成的影响，我们使用超高效液相色谱/四极杆飞行时间质谱对不同蔗糖水平处理的[植物名称未给出]进行了次生代谢产物分析。偏最小二乘判别分析和层次聚类分析表明，在50、150和300 mM蔗糖水平下，[植物名称未给出]的次生代谢产物谱存在显著差异。与50 mM蔗糖相比，槲皮素3 - β - d - 葡萄糖基 - (1→2) - β - d - 葡萄糖苷、6 - 甲氧基香豆素3 - 乙酸酯和3 - 羟基香豆素等3种黄酮类化合物以及包括6 - 甲氧基香豆素3 - 乙酸酯、金黄酮、鸢尾苷、黄酮醇3 - (6 - 丙二酰 - β - d - 葡萄糖苷)槲皮素3 - 葡萄糖苷和芦丁在内的19种黄酮类化合物的含量分别在150和300 mM蔗糖水平下增加，表明较高浓度的蔗糖会使[植物名称未给出]中黄酮类化合物积累。这是首次研究不同蔗糖水平下[植物名称未给出]中作为次生代谢产物的单个黄酮类化合物的变化，结果表明蔗糖作为一种抵御渗透胁迫的防御机制会导致某些黄酮类化合物的积累。
