解析从大豆乳清中分离出的W1产生胞外多糖的能力。
Decoding the Capability of W1 Isolated from Soybean Whey in Producing an Exopolysaccharide.
作者信息
Do Thi Bich Thuy, Tran Bao Khanh, Tran Thi Van Thi, Le Trung Hieu, Cnockaert Margo, Vandamme Peter, Nguyen Thi Hong Chuong, Nguyen Chinh Chien, Hong Sung Hyun, Kim Soo Young, Van Le Quyet
机构信息
Faculty of Engineering and Food Technology, Hue University of Agriculture and Forestry, Hue University, Thua Thien Hue 530000, Vietnam.
Hue University of Sciences, Hue University, Thua Thien Hue 530000, Vietnam.
出版信息
ACS Omega. 2020 Dec 17;5(51):33387-33394. doi: 10.1021/acsomega.0c05256. eCollection 2020 Dec 29.
This study aims at producing exopolysaccharides (EPS) from a lactic acid bacterial strain. The soybean whey-isolated W1 (EPS-W1), which belongs to genus , is identified using the phenylalanyl-tRNA sequencing method. Of all the examined strains, R-49778 (as numbered by BCCM/LMG Bacteria Collection, Ghent University, Belgium) showed the highest capability of producing exopolysaccharides. Structural characterization revealed a novel exopolysaccharide consisting of repeating units of →6)-d-Glcp-(1→; →3)-d-Manp-(1→; →3)-d-Glcp-(1→ and a branch of →6)-d-Manp-(1→; →2)-d-Glcp-(1→. This discovery opens up avenues for the production of EPS for food industries, functional foods, and biomedical applications.
本研究旨在从一株乳酸菌中生产胞外多糖（EPS）。采用苯丙氨酰 - tRNA测序方法鉴定了从大豆乳清中分离出的属于 属的W1（EPS - W1）。在所有检测的菌株中，R - 49778（由比利时根特大学BCCM/LMG细菌保藏中心编号）显示出最高的胞外多糖生产能力。结构表征揭示了一种新型胞外多糖，其由→6)-d-葡萄糖-(1→、→3)-d-甘露糖-(1→、→3)-d-葡萄糖-(1→的重复单元以及→6)-d-甘露糖-(1→; →2)-d-葡萄糖-(1→的分支组成。这一发现为食品工业、功能性食品和生物医学应用中胞外多糖的生产开辟了道路。
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