Dababat Abdelfattah, Arif Mian Abdur Rehman, Toktay Halil, Atiya Osameh, Shokat Sajid, E-Orakci Gul, Imren Mustafa, Singh Sukhwinder
International Maize and Wheat Improvement Centre, Turkey Office P.K. 39 Emek, 06511, Ankara, Turkey.
Nuclear Institute for Agriculture and Biology, Faisalabad, 38000, Pakistan.
J Appl Genet. 2021 Feb;62(1):93-98. doi: 10.1007/s13353-020-00607-y. Epub 2021 Jan 6.
Yield losses because of cereal cyst nematodes could be as high as 92%, causing a bottleneck for wheat production. An integrated approach (application of pesticides, crop rotation, and use of host resistance) is needed to manage this devastating pathogen where resistant cultivars are considered most effective. This necessitates the identification of nematode-resistant sources in the available germplasm. Here, we report on the genetic mapping of nematode resistance in 255 diverse prebreeding lines (PBLs) employing an association mapping strategy. Altogether, seven additive quantitative trait loci (QTL) were identified on chromosomes 1A, 2A, 2B, 2D, 3A, 6B, and 6D explaining a maximum of 9.42% phenotypic variation where at least five QTL (on chromosomes 2A, 2B, 2D, 6B, and 6D) are located on the same chromosomes that harbor the already known nematode resistance genes. Resistant PBLs carried Aegilops squarrosa (436) in their pedigree which could be the possible source of positive alleles. To add to it, better yield performance of the identified nematode-resistant lines under stress conditions indicates that the germplasm can provide both nematode resistance and high-yielding cultivars.
谷物胞囊线虫造成的产量损失可能高达92%，这给小麦生产带来了瓶颈。需要采取综合方法（施用农药、轮作和利用寄主抗性）来管理这种具有毁灭性的病原体，其中抗性品种被认为是最有效的。这就需要在现有的种质资源中鉴定线虫抗性来源。在此，我们报告了采用关联作图策略对255个不同的预育种系（PBL）进行线虫抗性基因定位的情况。总共在1A、2A、2B、2D、3A、6B和6D染色体上鉴定出7个加性数量性状位点（QTL），解释了最多9.42%的表型变异，其中至少有5个QTL（位于2A、2B、2D、6B和6D染色体上）位于与已知线虫抗性基因相同的染色体上。抗性PBL的系谱中含有粗山羊草（436），这可能是正向等位基因的来源。此外，在胁迫条件下鉴定出的抗线虫品系具有更好的产量表现，这表明该种质资源可以提供抗线虫和高产的品种。
