Millis R M, Ewii M C, Offiah G U, Hyde B P, Knight E M
Department of Zoology, Graduate School of Arts and Sciences, Howard University, Washington, D.C. 20059.
Pediatr Res. 1988 Jan;23(1):93-7. doi: 10.1203/00006450-198801000-00021.
Previous studies have shown that salicylates and protein-calorie malnutrition compromise immunological responses in humans and experimental animals. The present study compared the effects of prenatal normal and low protein diets, with and without aspirin-like drug treatments, on lymphocyte blastogenesis measured by tritiated thymidine uptake for DNA synthesis in splenic lymphocytes from pregnant rats and their offspring following stimulation with the mitogens concanavalin A, phytohemagglutinin, and pokeweed mitogen. Aspirin treatment was associated with increased lymphocyte thymidine uptake for blastogenesis in pregnant rats fed the normal protein control diet and their offspring. The phytohemagglutinin-stimulated increase detected in offspring lymphocytes could not be statistically guaranteed. A low protein diet alone and a normal protein diet combined with salicylamide treatment was associated with decreased blastogenesis in pregnant rats but not in their offspring. Salicylamide or aspirin combined with a low-protein diet decreased blastogenesis in both dams and their offspring. Aspirin combined with a normal protein diet did not adversely affect blastogenesis in either pregnant rats or their offspring. This study suggests that low dietary protein and aspirin-like drugs may independently decrease lymphocyte blastogenesis of pregnant rats and in combination they may also reduce lymphocyte blastogenesis in offspring. The significance of increased lymphocyte blastogenesis in both mothers and offspring following aspirin treatment of pregnant rats fed a normal protein diet is unclear.
以往的研究表明，水杨酸盐和蛋白质 - 热量营养不良会损害人类和实验动物的免疫反应。本研究比较了产前正常蛋白饮食和低蛋白饮食，以及有无阿司匹林样药物处理，对怀孕大鼠及其后代脾淋巴细胞在受到促细胞分裂剂刀豆球蛋白A、植物血凝素和商陆有丝分裂原刺激后，通过氚标记胸腺嘧啶核苷摄取来测量DNA合成的淋巴细胞增殖的影响。阿司匹林处理使喂食正常蛋白对照饮食的怀孕大鼠及其后代中，淋巴细胞增殖的胸腺嘧啶核苷摄取增加。在后代淋巴细胞中检测到的植物血凝素刺激增加在统计学上无法得到保证。单独的低蛋白饮食以及正常蛋白饮食与水杨酰胺处理相结合，会使怀孕大鼠的增殖降低，但对其后代无影响。水杨酰胺或阿司匹林与低蛋白饮食相结合，会使母鼠及其后代的增殖降低。阿司匹林与正常蛋白饮食相结合，对怀孕大鼠或其后代的增殖均无不利影响。本研究表明，低膳食蛋白和阿司匹林样药物可能会独立降低怀孕大鼠的淋巴细胞增殖，并且它们共同作用时也可能会降低后代的淋巴细胞增殖。在喂食正常蛋白饮食的怀孕大鼠接受阿司匹林处理后，母鼠和后代的淋巴细胞增殖增加的意义尚不清楚。
