微傅里叶变换红外光谱结合曲线拟合方法研究棉纤维发育过程中纤维素结晶度。

Micro-FTIR combined with curve fitting method to study cellulose crystallinity of developing cotton fibers.

机构信息

Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434023, China.

State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, The Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, China.

出版信息

Anal Bioanal Chem. 2021 Feb;413(5):1313-1320. doi: 10.1007/s00216-020-03094-6. Epub 2021 Jan 6.

DOI:10.1007/s00216-020-03094-6

PMID:33404744

Abstract

This study aimed to use micro-FTIR with transmission mode to investigate cellulose crystallinity of developing cotton fibers. Compared with ATR-FTIR method, we found that micro-FTIR can obtain more information of cellulose inside of the developing cotton fibers, especially in high wavenumber of 2800-3000 cm region. Combined with curve fitting method, a new IR crystallinity index (CI) method named wax crystallinity index (WCI) was introduced to evaluate the cellulose crystallinity in the development of cotton fibers based on the peak and area ratios of 2900 cm/2850 cm and 2900 cm/2920 cm. The obtained WCI values demonstrated an excellent coefficient of determination with X-ray diffraction (XRD) CI method with the value up to 0.99. This study suggested that micro-FTIR was an effective technique to qualitatively analyze the crystallinity in developing cotton fibers combined with curve fitting method.

摘要

本研究旨在采用透射模式的微傅里叶变换红外光谱（micro-FTIR）技术研究棉纤维发育过程中的纤维素结晶度。与衰减全反射傅里叶变换红外光谱（ATR-FTIR）方法相比，我们发现 micro-FTIR 可以获得更多关于发育中棉纤维内部纤维素的信息，特别是在 2800-3000 cm-1 的高波数区域。结合曲线拟合方法，我们引入了一种新的红外结晶度指数（CI）方法，称为蜡结晶度指数（WCI），该方法基于 2900 cm-1/2850 cm-1 和 2900 cm-1/2920 cm-1 峰和面积比来评估棉纤维发育过程中纤维素的结晶度。所得的 WCI 值与 X 射线衍射（XRD）CI 方法具有极好的决定系数，相关系数高达 0.99。本研究表明，micro-FTIR 结合曲线拟合方法是一种定性分析发育中棉纤维结晶度的有效技术。