Department of Restorative Dentistry, Dentistry Faculty, University of Chile, Santiago, Chile.
Institute of Research in Dental Sciences, Dentistry Faculty, University of Chile, Olivos 943, 8380492, Santiago, Metropolitan Region, Chile.
Clin Oral Investig. 2021 Jun;25(6):3823-3830. doi: 10.1007/s00784-020-03712-8. Epub 2021 Jan 6.
This study evaluated the effect of milk supplemented with Lactobacillus rhamnosus SP1 on the occurrence of caries and the salivary concentration of human β-defensin-3 (hβD-3) in preschool children with high caries risk.
A sample of 42 children was randomly assigned to two groups; children in the intervention group were given 150 mL of milk supplemented with 10 CFU/mL of Lactobacillus rhamnosus SP1, while children in the control group were given standard milk, for 10 months. The occurrence of dental caries was assessed using the International Caries Detection and Assessment System (ICDAS), and the concentration of hβD-3 was measured in unstimulated saliva using an ELISA test at baseline and after the intervention.
There was an increase in the number of teeth with carious lesions (d mft) in the control group, and this increase was statistically significant (p = 0.0489). The concentration of hβD-3 in saliva from the intervention group decreased from 597.91 to 126.29 pg/mL (p = 0.0061), unlike in the control group, where no change in hβD-3 salivary concentration was found.
These findings showed that regular intake of probiotic-supplemented milk in preschool children with high caries risk decreased the occurrence of caries and the salivary levels of hβD-3.
Our results suggest the need for developing and implementing probiotic supplementation, as adjuvants to the conventional treatments for caries and allow to considerate the salivary levels of hβD-3 as markers of oral tissue homeostasis.
本研究评估了富含鼠李糖乳杆菌 SP1 的牛奶对高龋风险学龄前儿童龋齿发生和唾液人β防御素-3（hβD-3）浓度的影响。
将 42 名儿童随机分为两组；干预组儿童饮用添加 10 CFU/mL 鼠李糖乳杆菌 SP1 的 150 mL 牛奶，而对照组儿童饮用标准牛奶，为期 10 个月。采用国际龋病检测和评估系统（ICDAS）评估龋齿发生情况，采用 ELISA 法检测基线和干预后未刺激唾液中 hβD-3 的浓度。
对照组牙齿龋损数（dmft）增加，差异有统计学意义（p = 0.0489）。干预组唾液中 hβD-3 的浓度从 597.91 降至 126.29 pg/mL（p = 0.0061），而对照组 hβD-3 唾液浓度无变化。
这些发现表明，高龋风险学龄前儿童定期摄入含益生菌的牛奶可减少龋齿的发生和唾液中 hβD-3 的水平。
我们的研究结果表明，需要开发和实施益生菌补充剂作为龋齿常规治疗的辅助手段，并考虑将唾液中 hβD-3 的水平作为口腔组织稳态的标志物。
