Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
Methods Mol Biol. 2021;2260:1-13. doi: 10.1007/978-1-0716-1182-1_1.
Here we present a protocol of interest to those who want to look at the functional consequences of decreasing expression of essential genes constitutively as well as being able to study the regulatory pathways of that essential gene by also deleting one or two additional genes. This allows epistasis relationships to be determined.
在这里，我们为那些希望观察持续降低必需基因表达的功能后果的人以及能够通过删除一个或两个额外基因来研究该必需基因的调控途径的人提供了一个感兴趣的方案。这允许确定上位性关系。
