使用全细胞亲和纯化技术鉴定真菌的宿主受体。
Identification of Host Receptors for Fungi Using Whole Cell Affinity Purification.
机构信息
Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, USA.
Lundquist Institute for Biomedical Innovation at Harbor-UCLA Medical Center, Torrance, CA, USA.
出版信息
Methods Mol Biol. 2021;2260:27-36. doi: 10.1007/978-1-0716-1182-1_3.
Abstract
Receptors on endothelial and epithelial cells often recognize molecules that are expressed by fungi, and only a limited number of these receptors have been identified to date. Here, we describe a method for identifying novel host cell receptors for fungi that uses intact organisms to precipitate biotin-labelled host cell membrane proteins, which are then detected by immunoblotting with an anti-biotin antibody. Presented here is the method to use for identification of membrane proteins that bind to C. albicans.
摘要
内皮细胞和上皮细胞上的受体通常能识别真菌表达的分子，但迄今为止，这些受体中只有少数被鉴定出来。在这里，我们描述了一种使用完整生物体沉淀生物素标记的宿主细胞膜蛋白的方法，然后用抗生物素抗体通过免疫印迹检测这些蛋白，从而鉴定真菌的新宿主细胞受体。本文介绍了一种用于鉴定与 C. albicans 结合的膜蛋白的方法。
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EphA2 is an epithelial cell pattern recognition receptor for fungal β-glucans.EphA2 是一种上皮细胞模式识别受体，可识别真菌β-葡聚糖。
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Als3 is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells.Als3是一种白色念珠菌入侵蛋白，它与钙黏蛋白结合并诱导宿主细胞进行内吞作用。
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