Ralph Benjamin, Sheppard Donald C
Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada.
Program in Infectious Diseases and Immunology in Global Health, Centre for Translational Biology, The Research Institute of the McGill University Health Center (RI-MUHC), Montréal, QC, Canada.
Methods Mol Biol. 2021;2260:215-224. doi: 10.1007/978-1-0716-1182-1_15.
In addition to causing acute invasive infections in immunocompromised patients, the mold Aspergillus fumigatus causes chronic infections in patients with chronic pulmonary conditions such as cystic fibrosis. Here we describe a non-lethal model of chronic pulmonary aspergillosis in which immunocompetent mice are endotracheally infected with A. fumigatus conidia embedded in agar beads. This approach results in the establishment of hyphal infection within the airways of mice for up to a 28-day period and is amenable to the study of innate and adaptive antifungal responses, fungal mutant strains, and antifungal agents.
除了在免疫功能低下的患者中引起急性侵袭性感染外，烟曲霉还会在患有慢性肺部疾病（如囊性纤维化）的患者中引起慢性感染。在此，我们描述了一种慢性肺曲霉病的非致死模型，其中免疫功能正常的小鼠通过气管内接种嵌入琼脂珠中的烟曲霉分生孢子进行感染。这种方法可使小鼠气道内建立长达28天的菌丝感染，适用于研究先天性和适应性抗真菌反应、真菌突变菌株及抗真菌药物。
