Edinburgh Genome Foundry, SynthSys, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2021;2229:167-174. doi: 10.1007/978-1-0716-1032-9_7.
Restriction digest analysis and Sanger sequencing are among the most commonly used techniques to check the sequence of synthetic DNA constructs. However, both require careful preparation to select restriction enzymes or DNA primers adapted to the expected constructs sequences. In projects involving manufacturing of large batches of synthetic constructs, the task can be tedious and error-prone. This chapter demonstrates the use of two free and open-source web applications providing fast and automated selection of enzymes and sequencing primers for DNA construct verification.
限制性内切酶分析和 Sanger 测序是最常用于检查合成 DNA 构建体序列的技术之一。然而，这两种技术都需要仔细准备，以选择适应预期构建体序列的限制性内切酶或 DNA 引物。在涉及制造大量合成构建体的项目中，这项任务可能会很繁琐且容易出错。本章演示了两种免费的开源网络应用程序的使用方法，这些应用程序可快速且自动地选择用于 DNA 构建体验证的酶和测序引物。
