Edinburgh Genome Foundry, SynthSys, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2021;2229:157-166. doi: 10.1007/978-1-0716-1032-9_6.
Type-2S restriction enzymes allow the routine assembly of large batches of synthetic constructs from individual genetic parts. However, design flaws in the part sequence can cause assembly failures, incurring troubleshooting costs and project delays. As a result, the careful design and checking of the assembly plan is often a bottleneck of large assembly projects, and may require computational support. This chapter demonstrates the use of two free and open-source web applications accelerating this task by automating genetic part design and simulating type-2S cloning to detect potential assembly issues.
2S 型限制酶允许从单个遗传元件常规组装大批合成构建体。然而，元件序列中的设计缺陷可能导致组装失败，从而增加故障排除成本和项目延迟。因此，精心设计和检查组装计划通常是大型组装项目的瓶颈，可能需要计算支持。本章演示了使用两个免费和开源的网络应用程序来加速该任务，通过自动化遗传元件设计和模拟 2S 型克隆来检测潜在的组装问题。
