Department of Engineering Mathematics, University of Bristol, Bristol, UK.
School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.
Methods Mol Biol. 2021;2229:205-219. doi: 10.1007/978-1-0716-1032-9_10.
Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.
最近，控制工程学在哺乳动物细胞生物学中的应用已经被用于精确调控基因表达。在这一章中，我们报告了主要的实验和计算方法，以使用微流控/显微镜平台在哺乳动物细胞中实现基因表达的自动反馈控制。
