Wang Shilei, Huang Qiaomu, Liu Xiangyu, Li Zhao, Yang Hao, Lu Zhong
Research Center for Environmental Ecology and Engineering, Key Laboratory for Green Chemical Process of Ministry of Education, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, No. 206, Guanggu first road, Wuhan 430073, PR China.
ACS Biomater Sci Eng. 2019 Apr 8;5(4):2030-2040. doi: 10.1021/acsbiomaterials.9b00118. Epub 2019 Mar 6.
The integration of nanomaterials with clinical therapeutic instruments is a promising approach to improve the effects of nanomaterials. We reported an efficient synergistic antibacterial strategy formed through the combination of Ag/ZnO nanocomposites with a light-emitting diode (LED) curing light, which is a commonly used small instrument in dental clinics. The as-designed integration depicted a significantly enhanced bactericidal effect on facultative anaerobic oral pathogen () both in planktonic and biofilm phases over a very short irradiation time (≤5 min). Further study showed that the combination of LED and Ag/ZnO nanocomposites induced more ·OH and ·O generation, which is responsible for the enhanced antibacterial activity. Moreover, this combination could destroy biofilm by killing the bacteria embedded within biofilm, inhibiting exopolysaccharide production and down-regulating the biofilm-related gene expression. Therefore, it is proposed that this combination could be applied in dental clinics to realize dental caries prevention and dental restoration simultaneously.
纳米材料与临床治疗器械的整合是提高纳米材料效果的一种有前景的方法。我们报道了一种通过将Ag/ZnO纳米复合材料与发光二极管（LED）固化灯相结合形成的高效协同抗菌策略，LED固化灯是牙科诊所常用的小型器械。所设计的这种整合在非常短的照射时间（≤5分钟）内，对兼性厌氧口腔病原体（）在浮游和生物膜阶段均表现出显著增强的杀菌效果。进一步研究表明，LED与Ag/ZnO纳米复合材料的组合诱导产生了更多的·OH和·O，这是抗菌活性增强的原因。此外，这种组合可以通过杀死嵌入生物膜中的细菌、抑制胞外多糖的产生以及下调生物膜相关基因的表达来破坏生物膜。因此，有人提出这种组合可应用于牙科诊所，以同时实现龋齿预防和牙齿修复。
