Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
BK21 FOUR Education and Research Team for Sustainable Food & Nutrition, Seoul National University, Seoul 08826, Korea.
Int J Mol Sci. 2021 Jun 8;22(12):6205. doi: 10.3390/ijms22126205.
The objective of this study was to determine reactive oxygen species (ROS) produced by fagopyrin F-rich fraction (FFF) separated from Tartary buckwheat flower extract exposed to lights and to investigate its antibacterial photodynamic inactivation (PDI) against and its biofilm. ROS producing mechanisms involving FFF with light exposure were determined using a spectrophotometer and a fluorometer. and its biofilm inactivation after PDI treatment of FFF using blue light (BL; 450 nm) were determined by plate count method and crystal violet assay, respectively. The biofilm destruction by ROS produced from FFF after exposure to BL was visualized using confocal laser scanning microscopy (CLSM) and field emission scanning electron microscope (FE-SEM). BL among 3 light sources produced type 1 ROS the most when applying FFF as a photosensitizer. FFF exposed to BL (5 and 10 J/cm) significantly more inhibited viability and biofilm formation than FFF without the light exposure ( < 0.05). In the PDI of FFF exposed to BL (10 J/cm), an apparent destruction of and its biofilm were observed by the CLSM and FE-SEM. Antibacterial PDI effect of FFF was determined for the first time in this study.
本研究旨在确定从苦荞花提取物中分离出的富含荞麦苦素 F 的富分（FFF）在暴露于光线下时产生的活性氧（ROS），并研究其对 及其生物膜的光动力抗菌灭活（PDI）作用。使用分光光度计和荧光计确定涉及光暴露的 FFF 的 ROS 产生机制。使用平板计数法和结晶紫测定法分别测定使用蓝光（BL；450nm）对 FFF 进行 PDI 处理后 和其生物膜的灭活情况。通过共聚焦激光扫描显微镜（CLSM）和场发射扫描电子显微镜（FE-SEM）观察 BL 暴露后 FFF 产生的 ROS 对生物膜的破坏情况。当将 FFF 用作光敏剂时，3 种光源中的 BL 产生的 1 型 ROS 最多。与未暴露于光的 FFF 相比，暴露于 BL（5 和 10 J/cm）的 FFF 显著抑制了 的活力和生物膜形成（<0.05）。在暴露于 BL 的 FFF 的 PDI 中（10 J/cm），通过 CLSM 和 FE-SEM 观察到对 和其生物膜的明显破坏。本研究首次确定了 FFF 的抗菌 PDI 作用。
