Li Dan, Yan Xiaoqian, Hu Yangnan, Liu Yun, Guo Rongrong, Liao Menghui, Shao Buwei, Tang Qilin, Guo Xing, Chai Renjie, Zhang Qi, Tang Mingliang
Key Laboratory for Developmental Genes and Human Disease, Ministry of Education, Institute of Life Sciences, Southeast University, Nanjing 210096, China.
Co-innovation Center of Neuroregeneration, Nantong University, Nantong 226001, China.
ACS Biomater Sci Eng. 2019 Mar 11;5(3):1561-1568. doi: 10.1021/acsbiomaterials.8b01231. Epub 2019 Feb 14.
Iridium(III) complexes have been shown to be promising probes in two-photon imaging to real-time track the transplanted cells in stem-cell-based therapy. Here, we report on polymeric nanocapsules loaded with red phosphorescence dye of bis(2-methyldibenzo[f,h]quinoxaline) (acetylacetonate) iridium(III) (Ir(MDQ)acac) with excellent stability created by the double emulsion method. The Ir(MDQ)acac nanocapsules present high biocompatibility and an efficient fluorescent labeling rate when incubated with cultured mouse neural stem cells (NSCs). More importantly, the Ir(MDQ)acac nanocapsules had both one- and two-photon imaging properties with stable phosphorescence lasting for 72 h. Furthermore, data from tracking in nude mice demonstrated that the photoluminescence from Ir(MDQ)acac nanocapsules in NSCs could be stably monitored for up to 21 days. Our data shed light on the potential clinical application of iridium complexes encapsulated in polymeric nanospheres for two-photon imaging in real-time tracking of the transplanted stem cells.
铱（III）配合物已被证明是双光子成像中用于实时追踪基于干细胞治疗中移植细胞的有前景的探针。在此，我们报道了通过双乳液法制备的负载双（2 - 甲基二苯并[f,h]喹喔啉）（乙酰丙酮）铱（III）（Ir(MDQ)acac）红色磷光染料且具有优异稳定性的聚合物纳米胶囊。当与培养的小鼠神经干细胞（NSCs）孵育时，Ir(MDQ)acac纳米胶囊具有高生物相容性和高效的荧光标记率。更重要的是，Ir(MDQ)acac纳米胶囊具有单光子和双光子成像特性，其磷光稳定持续72小时。此外，裸鼠体内追踪数据表明，NSCs中Ir(MDQ)acac纳米胶囊的光致发光可稳定监测长达21天。我们的数据为包裹在聚合物纳米球中的铱配合物在双光子成像实时追踪移植干细胞方面的潜在临床应用提供了线索。
