Peng Xuqi, Wang Bingquan, Yang Yu, Zhang Yihan, Liu Yonggang, He Yuan, Zhang Ce, Fan Haiming
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xuefu Street No. 1, Xi'an, 710127, China.
School of Chemical Engineering, Northwest University, Xuefu Street No. 1, Xi'an, 710069, China.
ACS Biomater Sci Eng. 2019 Mar 11;5(3):1635-1644. doi: 10.1021/acsbiomaterials.8b01630. Epub 2019 Feb 13.
Mitochondria-targeting nanotherapy receives great attention these days for its capacity in disrupting mitochondria function and inducing tumor cell apoptosis through external magnetic and optical stimulations. However, the effect is significantly diminished when applied to animal models. The key factors include environmental complexity in vivo and intrinsic protective features of tumor tissues. To address these obstacles and reduce expenses on drug screening, we herein introduce a methodology for producing millimeter-sized spheroids with structural and functional characteristics of tumor tissues in vivo. The necessity of spheroid as a liver tumor model is demonstrated by comparing the effect of TPP-SPIONs (triphenylphosphonium cation-superparamagnetic iron oxide nanoparticles) on monolayer-cultured HepG2 cells and spheroids. Our study reveals that large-scale spheroid, in contrast to monolayer cells, reflects more in vivo tumor characters and is less responsive to TPP-SPIONs during magnetic hyperthermia treatment.
近年来，线粒体靶向纳米疗法备受关注，因为它能够通过外部磁刺激和光刺激破坏线粒体功能并诱导肿瘤细胞凋亡。然而，当应用于动物模型时，这种效果会显著减弱。关键因素包括体内环境的复杂性和肿瘤组织的固有保护特性。为了解决这些障碍并减少药物筛选的费用，我们在此介绍一种方法，用于制备具有体内肿瘤组织结构和功能特征的毫米级球体。通过比较TPP-SPIONs（三苯基膦阳离子-超顺磁性氧化铁纳米颗粒）对单层培养的HepG2细胞和球体的作用，证明了球体作为肝肿瘤模型的必要性。我们的研究表明，与单层细胞相比，大规模球体更能反映体内肿瘤特征，并且在磁热疗过程中对TPP-SPIONs的反应较小。
