Li Xiaoting, Wang Xinhui, Hua Mingli, Yu Haohan, Wei Shaohua, Wang Ao, Zhou Jiahong
College of Life Sciences, Jiangsu Key Laboratory of Biofunctional Materials, Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials, Key Laboratory of Applied Photochemistry, Nanjing Normal University, Wenyuan Road, Nanjing, Jiangsu 210023, China.
Key Lab of Biomass Energy and Material, Jiangsu Province, National Engineering Lab. For Biomass Chemical Utilization, Key and Open Lab. of Forest Chemical Engineering, SFA, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, Suojin Fifth Village, Nanjing, Jiangsu 210042, China.
ACS Biomater Sci Eng. 2019 May 13;5(5):2399-2408. doi: 10.1021/acsbiomaterials.9b00478. Epub 2019 Apr 26.
Base-pairing is stable under physiological temperature but broken by heating, which is the basic mechanism for nucleic acid amplification in biology. In this manuscript, a simple controlled-drug-release system was prepared on the basis of this rule and its in vivo activity was studied. Poly adenine (poly A), the tail of the synthesized RNA chain, was exploited as gatekeeper of thymine (T)-modified mesoporous silica nanoparticles (MSN-T) based on the simple A-T base-pairing rules. The gate keeper maintains stability to avoid drug (chemotherapy and photothermal therapy drugs) release during the delivery process but is effectively removed by a photothermal effect to trigger drug release at the tumor tissue by near-infrared (NIR) laser irradiation. In vitro and in vivo experiments indicated that the prepared nanomedicine could effectively suppress tumor growth and activate antitumor immunity.
碱基配对在生理温度下是稳定的，但加热会使其断裂，这是生物学中核酸扩增的基本机制。在本论文中，基于这一规则制备了一种简单的控释系统，并研究了其体内活性。基于简单的A-T碱基配对规则，合成RNA链的尾巴聚腺嘌呤（poly A）被用作胸腺嘧啶（T）修饰的介孔二氧化硅纳米颗粒（MSN-T）的“守门人”。“守门人”保持稳定性以避免药物（化疗和光热治疗药物）在递送过程中释放，但通过光热效应有效地去除，以通过近红外（NIR）激光照射触发肿瘤组织处的药物释放。体外和体内实验表明，所制备的纳米药物可以有效地抑制肿瘤生长并激活抗肿瘤免疫。
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