Wang Juan, Zheng Wei, Chen Liang, Zhu Tonghe, Shen Wei, Fan Cunyi, Wang Hongjun, Mo Xiumei
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Department of Orthopaedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
ACS Biomater Sci Eng. 2019 May 13;5(5):2444-2456. doi: 10.1021/acsbiomaterials.8b01564. Epub 2019 Apr 23.
Peripheral nerve regeneration and functional recovery remain a significant clinical challenge. Biomaterials that can regulate biological behavior of Schwann cell (SC) and promote neural cell differentiation are beneficial for nerve regeneration and functional recovery. Graphene oxide (GO), as a bioactive nanomaterial, has attracted great attention in biomedical applications. In this study, the possibility of combining the excellent physicochemical properties of GO with nanofiber to develop a bioactive scaffold for nerve regeneration were explored. Briefly, GO was coated on the F/PLCL scaffolds. To demonstrate the potentials of this platform, in vitro and in vivo studies toward nerve regeneration were carried out. In vitro, GO-coated scaffolds could enhance SC biological behaviors including migration, proliferation, and myelination. The secretions from SCs cultured on GO-F/PLCL scaffolds could induce PC12 cells differentiation. Furthermore, GO-coated nanofibrous scaffolds proved to up-regulate focal adhesion kinase (FAK) expression of PC12 cell. In vivo, GO-F/PLCL nerve conduits could successfully repair a 10 mm sciatic nerve defect. These findings suggest that GO-based scaffolds efficiently modulate cell functions and promote nerve regeneration, indicating their potential for nerve regeneration applications.
周围神经再生和功能恢复仍然是一项重大的临床挑战。能够调节雪旺细胞（SC）生物学行为并促进神经细胞分化的生物材料有利于神经再生和功能恢复。氧化石墨烯（GO）作为一种生物活性纳米材料，在生物医学应用中备受关注。在本研究中，探讨了将GO的优异理化性质与纳米纤维相结合以开发用于神经再生的生物活性支架的可能性。简而言之，将GO涂覆在F/PLCL支架上。为了证明该平台的潜力，进行了针对神经再生的体外和体内研究。在体外，GO涂覆的支架可以增强SC的生物学行为，包括迁移、增殖和髓鞘形成。在GO-F/PLCL支架上培养的SCs的分泌物可以诱导PC12细胞分化。此外，GO涂覆的纳米纤维支架被证明可以上调PC12细胞的粘着斑激酶（FAK）表达。在体内，GO-F/PLCL神经导管可以成功修复10毫米的坐骨神经缺损。这些发现表明，基于GO的支架能够有效调节细胞功能并促进神经再生，显示出它们在神经再生应用中的潜力。
