Ling Jue, He Chang, Zhang Shuxuan, Zhao Yahong, Zhu Meifeng, Tang Xiaoxuan, Li Qiaoyuan, Xu Liming, Yang Yumin
Key Laboratory of Neuroregeneration, Ministry of Education and Jiangsu Province, Co-Innovation Center of Tissue Engineering and Nerve Injury Repair, Nantong University, Nantong, China.
College of Life Sciences, Nankai University, Tianjin, China.
Front Bioeng Biotechnol. 2023 Dec 7;11:1308761. doi: 10.3389/fbioe.2023.1308761. eCollection 2023.
Peripheral nerve injury (PNI) is a neurological disorder caused by trauma that is frequently induced by accidents, war, and surgical complications, which is of global significance. The severity of the injury determines the potential for lifelong disability in patients. Artificial nerve scaffolds have been investigated as a powerful tool for promoting optimal regeneration of nerve defects. Over the past few decades, bionic scaffolds have been successfully developed to provide guidance and biological cues to facilitate Schwann cell myelination and orientated axonal growth. Numerous assessment techniques have been employed to investigate the therapeutic efficacy of nerve scaffolds in promoting the growth of Schwann cells and axons upon the bioactivities of distinct scaffolds, which have encouraged a greater understanding of the biological mechanisms involved in peripheral nerve development and regeneration. However, it is still difficult to compare the results from different labs due to the diversity of protocols and the availability of innovative technologies when evaluating the effectiveness of novel artificial scaffolds. Meanwhile, due to the complicated process of peripheral nerve regeneration, several evaluation methods are usually combined in studies on peripheral nerve repair. Herein, we have provided an overview of the evaluation methods used to study the outcomes of scaffold-based therapies for PNI in experimental animal models and especially focus on Schwann cell functions and axonal growth within the regenerated nerve.
周围神经损伤（PNI）是一种由创伤引起的神经疾病，常见于事故、战争和手术并发症，具有全球意义。损伤的严重程度决定了患者终身残疾的可能性。人工神经支架已被研究作为促进神经缺损最佳再生的有力工具。在过去几十年中，已成功开发出仿生支架，以提供引导和生物信号，促进施万细胞髓鞘形成和轴突定向生长。众多评估技术已被用于研究神经支架在不同支架生物活性作用下促进施万细胞和轴突生长的治疗效果，这有助于更深入地了解周围神经发育和再生所涉及的生物学机制。然而，在评估新型人工支架的有效性时，由于方案的多样性和创新技术的可用性，不同实验室的结果仍难以比较。同时，由于周围神经再生过程复杂，在周围神经修复研究中通常会结合几种评估方法。在此，我们概述了用于研究实验动物模型中基于支架的PNI治疗结果的评估方法，尤其关注再生神经内的施万细胞功能和轴突生长。
