Kucko Nathan W, de Lacerda Schickert Sónia, Sobral Marques Tomás, Herber Ralf-Peter, van den Beuken Jeroen J J P, Zuo Yi, Leeuwenburgh Sander C G
Department of Regenerative Biomaterials, Radboud University Medical Center, Philips van Leydenlaan 25 6525 EX, Nijmegen, The Netherlands.
CAM Bioceramics B.V., Zernikedreef 6 2333 CL, Leiden, The Netherlands.
ACS Biomater Sci Eng. 2019 May 13;5(5):2491-2505. doi: 10.1021/acsbiomaterials.9b00226. Epub 2019 Apr 30.
Injectable, self-setting calcium phosphate cements (CPCs) are favorable bone substitutes due to their osteocompatibility. However, due to their brittleness and low toughness, their clinical application is limited to non-load-bearing sites. The incorporation of poly(vinyl alcohol) (PVA) fibers into cementitious materials is a successful strategy in civil engineering for improving the mechanical performance of cements. However, PVA fibers in particular have not yet been applied to reinforce CPCs. Therefore, the aim of this study is to investigate the effect of PVA fibers on the mechanical properties of CPCs. Second, the cytocompatibility of these fibers is studied using cell culture tests. Finally, the osteocompatibility of PVA fiber-reinforced CPCs is studied after a 6 and 12 week implantation period in the femoral condyle of rabbits. Results reveal that the incorporation of PVA fibers into CPCs is a highly effective strategy to strengthen and toughen CPCs, since the flexural strength and toughness of CPCs increased by more than 3-fold and 435-fold, respectively, upon reinforcement with PVA fibers. cytocompatibility tests indicate that PVA fibers are cytocompatible, which is further confirmed by the results that show that PVA fibers do not compromise the excellent osteocompatibility of CPCs.
可注射、自固化磷酸钙骨水泥（CPCs）因其骨相容性而成为理想的骨替代物。然而，由于其脆性和低韧性，其临床应用仅限于非承重部位。在土木工程中，将聚乙烯醇（PVA）纤维掺入胶凝材料是提高水泥力学性能的成功策略。然而，特别是PVA纤维尚未应用于增强CPCs。因此，本研究的目的是研究PVA纤维对CPCs力学性能的影响。其次，通过细胞培养试验研究这些纤维的细胞相容性。最后，在兔股骨髁植入6周和12周后，研究PVA纤维增强CPCs的骨相容性。结果表明，将PVA纤维掺入CPCs是增强和增韧CPCs的高效策略，因为用PVA纤维增强后，CPCs的抗弯强度和韧性分别提高了3倍多和435倍。细胞相容性测试表明PVA纤维具有细胞相容性，这进一步得到了结果的证实，结果表明PVA纤维不会损害CPCs优异的骨相容性。
