Chen Shitian, Zhu Ling, Wen Wei, Lu Lu, Zhou Changren, Luo Binghong
Biomaterial Research Laboratory, Department of Material Science and Engineering, College of Chemistry and Materials, Jinan University, Guangzhou 510632, PR China.
Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Guangzhou 510632, PR China.
ACS Biomater Sci Eng. 2019 May 13;5(5):2506-2518. doi: 10.1021/acsbiomaterials.9b00254. Epub 2019 May 2.
Quercetin (Qu), a bioflavonoid, has been reported to positively affect bone metabolism. For the first time, Qu with different concentrations was utilized to functionalize 3D-printed poly(l-lactide) (PLLA) scaffold with the aid of a polydopamine (PDA) layer through a convenient and effective way in this study. Results revealed that the coexistence of PDA and Qu can capacitate the 3D-printed PLLA scaffold to possess rougher surface, as well as better hydrophilicity and compressive properties. The resulting PDA- and Qu-modified PLLA scaffolds (Qu/PD-PLLA) can sustainably release Qu to some extent, which is more beneficial to the proliferation and attachment of MC3T3-E1 cells, upregulating ALP activity and calcium nodules as well as promoting the expression of related osteogenic genes and proteins. More significantly, such a positive impact of the Qu on the cell affinity and osteogenic activity played in a dose-dependent manner. This study revealed the potential of the 3D-printed Qu/PD-PLLA scaffolds with a certain amount of Qu as bone-repair materials.
槲皮素（Qu）是一种生物类黄酮，据报道对骨代谢有积极影响。在本研究中，首次通过一种简便有效的方法，借助聚多巴胺（PDA）层，用不同浓度的槲皮素对3D打印的聚左旋乳酸（PLLA）支架进行功能化处理。结果表明，PDA和Qu的共存可使3D打印的PLLA支架表面更粗糙，具有更好的亲水性和压缩性能。所得的PDA和Qu修饰的PLLA支架（Qu/PD-PLLA）能在一定程度上持续释放Qu，这更有利于MC3T3-E1细胞的增殖和附着，上调碱性磷酸酶（ALP）活性和钙结节，以及促进相关成骨基因和蛋白质的表达。更显著的是，Qu对细胞亲和力和成骨活性的这种积极影响呈剂量依赖性。本研究揭示了含有一定量Qu的3D打印Qu/PD-PLLA支架作为骨修复材料的潜力。
