Rubio Natalie R, Fish Kyle D, Trimmer Barry A, Kaplan David L
Department of Biomedical Engineering, Tufts University, Science & Technology Center, 4 Colby Street, Medford, Massachusetts 02155, United States.
Department of Biology, Tufts University, 200 Boston Avenue #4700, Medford, Massachusetts 02155, United States.
ACS Biomater Sci Eng. 2019 Feb 11;5(2):1071-1082. doi: 10.1021/acsbiomaterials.8b01261. Epub 2019 Jan 15.
Tissue engineering is primarily associated with medical disciplines, and research has thus focused on mammalian cells. For applications where clinical relevance is not a constraint, it is useful to evaluate the potential of alternative cell sources to form tissues in vitro. Specifically, skeletal muscle tissue engineering for bioactuation and cultured foods could benefit from the incorporation of invertebrate cells because of their less stringent growth requirements and other versatile features. Here, we used a muscle cell line to demonstrate the benefits of insect cells relative to those derived from vertebrates. The cells were adapted to serum-free media, transitioned between adherent and suspension cultures, and manipulated with hormones. Furthermore, we analyzed edible scaffolds to support cell adhesion and assayed cellular protein and minerals to evaluate nutrition potential. The insect muscle cells exhibited advantageous growth patterns and hold unique functionality for tissue engineering applications beyond the medical realm.
组织工程主要与医学学科相关，因此研究集中在哺乳动物细胞上。对于临床相关性不是限制因素的应用，评估替代细胞来源在体外形成组织的潜力是有用的。具体而言，用于生物驱动和 cultured foods（此处cultured foods不太明确准确含义，可推测为培养食品）的骨骼肌组织工程可以受益于无脊椎动物细胞的加入，因为它们的生长要求不那么严格以及具有其他多种特性。在这里，我们使用一种肌肉细胞系来证明昆虫细胞相对于脊椎动物来源细胞的优势。这些细胞适应了无血清培养基，在贴壁培养和悬浮培养之间转换，并通过激素进行调控。此外，我们分析了可食用支架以支持细胞黏附，并检测了细胞蛋白质和矿物质以评估营养潜力。昆虫肌肉细胞表现出有利的生长模式，并在医学领域之外的组织工程应用中具有独特的功能。
