Abelseth Emily, Abelseth Laila, De la Vega Laura, Beyer Simon T, Wadsworth Samuel J, Willerth Stephanie M
Aspect Biosystems, 1781 W 75th Avenue, Vancouver, British Columbia V6P 6P2, Canada.
ACS Biomater Sci Eng. 2019 Jan 14;5(1):234-243. doi: 10.1021/acsbiomaterials.8b01235. Epub 2018 Dec 6.
3D bioprinting offers the opportunity to automate the process of tissue engineering, which combines biomaterial scaffolds and cells to generate substitutes for diseased or damaged tissues. These bioprinting methods construct tissue replacements by positioning cells encapsulated in bioinks into specific locations in the resulting constructs. Human induced pluripotent stem cells (hiPSCs) serve as an important tool when engineering neural tissues. These cells can be expanded indefinitely and differentiated into the cell types found in the central nervous systems, including neurons. One common method for differentiating hiPSCs into neural tissue requires the formation of aggregates inside of defined diameter microwells cultured in chemically defined media. However, 3D bioprinting of such hiPSC-derived aggregates has not been previously reported in the literature, as it requires the development of specialized bioinks for supporting cell survival and differentiation into mature neural phenotypes. Here we detail methods including preparing base material components of the bioink, producing the bioink, and the steps involved in printing 3D neural tissues derived from hiPSC-derived neural aggregates using Aspect Biosystems' novel RX1 printer and their lab-on-a-printer (LOP) technology.
3D生物打印为组织工程过程的自动化提供了契机，组织工程将生物材料支架和细胞结合起来，以生成用于替代患病或受损组织的替代品。这些生物打印方法通过将封装在生物墨水中的细胞定位到所得构建物的特定位置来构建组织替代物。在构建神经组织时，人类诱导多能干细胞（hiPSC）是一种重要工具。这些细胞可以无限扩增，并分化为中枢神经系统中发现的细胞类型，包括神经元。将hiPSC分化为神经组织的一种常见方法是在化学限定培养基中培养的限定直径微孔内形成聚集体。然而，此前文献中尚未报道过对这种源自hiPSC的聚集体进行3D生物打印，因为这需要开发专门的生物墨水来支持细胞存活并分化为成熟的神经表型。在此，我们详细介绍了一些方法，包括制备生物墨水的基础材料成分、生产生物墨水，以及使用Aspect Biosystems公司的新型RX1打印机及其打印机上实验室（LOP）技术打印源自hiPSC神经聚集体的3D神经组织所涉及的步骤。
