State Key Laboratory of Petroleum Resources and Prospectng, Beijing Key Laoratory of Petroleum Pollution and Control, Research Centre for Geomicrobial Resources and Application, Institute of Unconventional Oil and Gas Science and Technology, China University of Petroleum, Beijing, PR China.
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, PR China.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004618.
A novel sulphate-reducing, Gram-stain-negative, anaerobic strain, isolate XJ01, recovered from production fluid at the LiaoHe oilfield, PR China, was the subject of a polyphasic study. The isolate together with NCIMB 9442 and DSM 5308 formed a distinct, well-supported clade in the 16S rRNA gene tree. The taxonomic status of the clade was underscored by complementary phenotypic data. The three isolates comprising the clade formed distinct phyletic branches and were distinguished using a combination of physiological features and by low average nucleotide identity and digital DNA-DNA hybridization values. Consequently, it is proposed that isolate XJ01 represents a novel genus and species for which the name gen. nov., sp. nov. is proposed with the type strain XJ01 (=CGMCC 1.5227=DSM 107637). It is also proposed that and be reclassified as comb. nov. and comb. nov., respectively.
从中国辽河油田生产液中分离到一株硫酸盐还原、革兰氏阴性、厌氧的新菌株 XJ01,对其进行了多相研究。该菌株与 NCIMB 9442 和 DSM 5308 一起在 16S rRNA 基因树中形成了一个独特的、有充分支持的分支。该分支的分类学地位得到了补充表型数据的强调。组成该分支的三个菌株形成了不同的系统发育分支,并通过生理特征的组合以及低平均核苷酸身份和数字 DNA-DNA 杂交值来区分。因此,建议将菌株 XJ01 代表一个新的属和种,建议使用新的属名和种名,即 gen. nov.,sp. nov.,其模式菌株为 XJ01 (=CGMCC 1.5227=DSM 107637)。还建议将 和 重新分类为 comb. nov. 和 comb. nov.,分别。