Sohrabi Amir, Bassam-Tolami Fatemeh, Imani Mohsen
Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Nobels väg 12A, Solna Campus, PO Box 171 77, Stockholm, Sweden.
Department of Biology, Tehran Shargh Branch, Payame Noor University, Tehran, Iran.
J Obstet Gynaecol India. 2020 Dec;70(6):503-509. doi: 10.1007/s13224-020-01363-z. Epub 2020 Aug 24.
HPV genotypes are the most common etiological factor for genital neoplasia. It would appear that sexually transmitted infections accompanied with HPV genotypes might have synergistic interactions in cancer progression. The genetic polymorphisms are involved in metabolizing carcinogens which may contribute to the susceptibility of developing genital cancers by less efficient or overly down metabolic pathways and cell signaling. MTHFR polymorphisms are related to several metabolic disorders and human cancers. We investigated the contribution of MTHFR 1298 and MTHFR 677 polymorphisms as potential risk factors for outcomes with HPV genotypes and STIs in Iranian population.
As a case-control study, MTHFR A1298C and C677T were assessed for SNPs analysis using a PCR-RFLP assay in 50 cervical intraepithelial neoplasia (CIN) cases, 98 HPV-positive subjects and 47 non-cancerous/non-HPV patients as healthy controls.
Finding suggested a significant association between the MTHFR 1298 CC polymorphisms (OR = 3.5, 95% CI = 1.13-10.82, ≤ 0.05) in women with CIN as compared to non-cancerous/non-HPV subjects. There was not a significant difference of MTHFR 677 between outcomes.
It would seem MTHFR 1298 CC is more likely to be a potential risk factor for HPV-cervical cancer progression. Consequences support further attempts to understand the clinical manifestations of neoplasia related to genital infections and gene mutations.
人乳头瘤病毒（HPV）基因型是生殖器肿瘤最常见的病因。似乎伴有HPV基因型的性传播感染在癌症进展中可能具有协同相互作用。基因多态性参与致癌物的代谢，这可能通过代谢途径和细胞信号转导效率低下或过度下调而导致患生殖器癌的易感性。亚甲基四氢叶酸还原酶（MTHFR）多态性与几种代谢紊乱和人类癌症有关。我们调查了MTHFR 1298和MTHFR 677多态性作为伊朗人群中HPV基因型和性传播感染结果的潜在危险因素的作用。
作为一项病例对照研究，采用聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）对50例宫颈上皮内瘤变（CIN）病例、98例HPV阳性受试者和47例非癌/非HPV患者作为健康对照进行MTHFR A1298C和C677T单核苷酸多态性分析。
研究结果表明，与非癌/非HPV受试者相比，CIN女性中MTHFR 1298 CC多态性（比值比[OR]=3.5，95%可信区间[CI]=1.13-10.82，P≤0.05）有显著相关性。不同结果之间MTHFR 677无显著差异。
似乎MTHFR 1298 CC更有可能是HPV宫颈癌进展的潜在危险因素。研究结果支持进一步努力了解与生殖器感染和基因突变相关肿瘤形成的临床表现。
