Department of Molecular Biology, Research Center of Health Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.
Sepand Pathobiology Laboratory, Iran.
J Infect Public Health. 2017 Nov-Dec;10(6):730-733. doi: 10.1016/j.jiph.2016.10.006. Epub 2017 Feb 16.
Frequency of cervical cancer related to Human Papilloma Virus (HPV) has increased remarkably in less-developed countries. Hence, applying capable diagnostic methods is urgently needed, as is having a therapeutic strategy as an effective step for cervical cancer prevention. The aim of this study was to investigate the prevalence of various multi-type HPV infection patterns and their possible rising incidence in women with genital infections. This descriptive study was conducted on women who attended referral clinical laboratories in Tehran for genital infections from January 2012 until December 2013. A total of 1387 archival cervical scraping and lesion specimens were collected from referred women. HPV genotyping was performed using approved HPV commercial diagnostic technologies with either INNO-LiPA HPV or Geno Array Test kits. HPV was positive in 563 cases (40.59%) with mean age of 32.35±9.96. Single, multiple HPV genotypes and untypable cases were detected in 398 (70.69%), 160 (28.42%) and 5 (0.89%) cases, respectively. Multiple HPV infections were detected in 92 (57.5%), 42 (26.2%), 17 (10.6%) and 9 (5.7%) cases as two, three, four and five or more genotypes, respectively. The prevalence of 32 HPV genotypes was determined one by one. Seventeen HPV genotypes were identified in 95.78% of all positive infections. Five dominant genotypes, HPV6, 16, 53, 11 and 31, were identified in a total of 52.35%of the HPV positive cases. In the present study, we were able to evaluate the rate of multiple HPV types in genital infections. Nevertheless, it is necessary to evaluate the role of the dominant HPV low-risk types and the new probably high-risk genotypes, such as HPV53, in the increasing incidences of genital infections.
在欠发达国家，宫颈癌与人类乳头瘤病毒（HPV）的相关性显著增加。因此，迫切需要应用有效的诊断方法，同时还需要制定治疗策略，作为宫颈癌预防的有效步骤。本研究旨在调查女性生殖道感染中多种多型 HPV 感染模式的流行情况及其可能的上升趋势。这是一项描述性研究，于 2012 年 1 月至 2013 年 12 月期间对因生殖道感染就诊于德黑兰转诊临床实验室的女性进行。共收集了 1387 例存档的宫颈刮片和病变标本。采用 INNO-LiPA HPV 或 GenoArray 试剂盒等批准的 HPV 商业诊断技术对 HPV 进行基因分型。HPV 阳性 563 例（40.59%），平均年龄 32.35±9.96 岁。单型、多型和无法定型 HPV 基因型的病例分别为 398 例（70.69%）、160 例（28.42%）和 5 例（0.89%）。多型 HPV 感染分别为 92 例（57.5%）、42 例（26.2%）、17 例（10.6%）和 9 例（5.7%），分别为两种、三种、四种和五种或更多种基因型。逐一确定了 32 种 HPV 基因型的流行情况。所有阳性感染中，有 17 种 HPV 基因型。5 种主要基因型 HPV6、16、53、11 和 31 在 HPV 阳性病例中占 52.35%。在本研究中，我们能够评估生殖道感染中多种 HPV 类型的发生率。然而，有必要评估 HPV 低危型主要基因型和 HPV53 等新的可能高危基因型在生殖道感染发病率上升中的作用。
